Nucleoplasmic Nup98 controls gene expression by regulating a DExH/D-box protein

The nucleoporin Nup98 has been linked to the regulation of transcription and RNA metabolism, 1 - 3 but the mechanisms by which Nup98 contributes to these processes remains largely undefined. Recently, we uncovered interactions between Nup98 and several DExH/D-box proteins (DBPs), a protein family we...

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Bibliographic Details
Published in:Nucleus (Austin, Tex.) Tex.), 2018-01, Vol.9 (1), p.1-8
Main Authors: Capitanio, Juliana S., Montpetit, Ben, Wozniak, Richard W.
Format: Article
Language:English
Subjects:
DDX21
DDX3
DDX5
DExD/H-box helicases
DHX9
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FG-Nup
gene expression regulation
nuclear pore complex
nucleoporins
Nup98
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Summary:The nucleoporin Nup98 has been linked to the regulation of transcription and RNA metabolism, 1 - 3 but the mechanisms by which Nup98 contributes to these processes remains largely undefined. Recently, we uncovered interactions between Nup98 and several DExH/D-box proteins (DBPs), a protein family well-known for modulating gene expression and RNA metabolism. 4 - 6 Analysis of Nup98 and one of these DBPs, DHX9, showed that they directly interact, their association is facilitated by RNA, and Nup98 binding stimulates DHX9 ATPase activity. 7 Furthermore, these proteins were dependent on one another for their proper association with a subset of gene loci to control transcription and modulate mRNA splicing. 7 On the basis of these observations, we proposed that Nup98 functions to regulate DHX9 activity within the nucleoplasm. 7 Since Nup98 is associated with several DBPs, regulation of DHX9 by Nup98 may represent a paradigm for understanding how Nup98, and possibly other FG-Nup proteins, could direct the diverse cellular activities of multiple DBPs.
ISSN:1949-1034
1949-1042
DOI:10.1080/19491034.2017.1364826