Enzymatic logic calculation systems based on solid-state electrochemiluminescence and molecularly imprinted polymer film electrodes

• Published in: Biosensors & bioelectronics 2018-02, Vol.100, p.326-332
• Main Authors: Lian, Wenjing, Liang, Jiying, Shen, Li, Jin, Yue, Liu, Hongyun
• Format: Article
• Language: English
• Subjects: Ampicillin - analysis; Anti-Bacterial Agents - analysis; Biocatalysis; Biomacromolecular logic gates; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Electrochemical Techniques - instrumentation; Electrochemical Techniques - methods; Electrochemiluminescence; Electrodes; Equipment Design; Ferrous Compounds - chemistry; Glucose - chemistry; Glucose oxidase; Glucose Oxidase - chemistry; Luminescent Agents - chemistry; Luminol - chemistry; Molecular Imprinting - instrumentation; Molecular Imprinting - methods; Molecularly imprinted polymer films; Multi-valued outputs; Polymers - chemistry; Pyrroles - chemistry
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2017.09.023

The molecularly imprinted polymer (MIP) films were electropolymerized on the surface of Au electrodes with luminol and pyrrole (PY) as the two monomers and ampicillin (AM) as the template molecule. The electrochemiluminescence (ECL) intensity peak of polyluminol (PL) of the AM-free MIP films at 0.7V vs Ag/AgCl could be greatly enhanced by AM rebinding. In addition, the ECL signals of the MIP films could also be enhanced by the addition of glucose oxidase (GOD)/glucose and/or ferrocenedicarboxylic acid (Fc(COOH)2) in the testing solution. Moreover, Fc(COOH)2 exhibited cyclic voltammetric (CV) response at the AM-free MIP film electrodes. Based on these results, a binary 3-input/6-output biomolecular logic gate system was established with AM, GOD and Fc(COOH)2 as inputs and the ECL responses at different levels and CV signal as outputs. Some functional non-Boolean logic devices such as an encoder, a decoder and a demultiplexer were also constructed on the same platform. Particularly, on the basis of the same system, a ternary AND logic gate was established. The present work combined MIP film electrodes, the solid-state ECL, and the enzymatic reaction together, and various types of biomolecular logic circuits and devices were developed, which opened a novel avenue to construct more complicated bio-logic gate systems. •Combining MIP, solid-state ECL with enzymatic reaction to fabricate biomolecular logic systems.•Successfully constructing a more complicated binary logic gate and a ternary one.•AM acting both as a template and a promoter to enhance the ECL response of PL.•Increasing the output numbers by the ECL intensity dependent on different mechanisms.