Uliginosibacterium sangjuense sp. nov., isolated from sediment of the Nakdong River

• Published in: Antonie van Leeuwenhoek 2018-02, Vol.111 (2), p.259-264
• Main Authors: Han, Ji-Hye, Baek, Kiwoon, Ahn, Young-Hee, Lee, Wook Jae, Lee, Mi-Hwa
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Coenzyme Q8; Deoxyribonucleic acid; DNA; Fatty acids; Flagella; Gene sequencing; Life Sciences; Lipids; Medical Microbiology; Microbiology; New species; Next-generation sequencing; Original Paper; Phosphatidylethanolamine; Phosphatidylglycerol; Phylogenetics; Phylogeny; Plant Sciences; Quinones; Rivers; rRNA 16S; Soil Science & Conservation; Ubiquinone
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-017-0946-z

A Gram-negative, aerobic, motile by flagella, and light yellow bacterium, designated SS1-76 T , was isolated from sediment of the Nakdong River in Sangju-si, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate SS1-76 T belongs to the genus Uliginosibacterium of the family Rhodocyclaceae , exhibiting high sequence similarity with the type strains of Uliginosibacterium gangwonense 5YN10-9 T (96.0%) and Uliginosibacterium paludis KBP-13 T (94.9%). Strain SS1-76 T contains ubiquinone-8 as a respiratory quinone and summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c ), C 16:0 , and C 14:0 as major fatty acids. The cellular polar lipids are composed of phosphatidylethanolamine, phosphatidylglycerol, and unidentified aminophospholipids. The DNA G+C content was 65.3 mol%. Phenotypic, chemotaxonomic, and phylogenetic evidence clearly indicated that strain SS1-76 T represents a novel species of the genus Uliginosibacterium , for which the name Uliginosibacterium sangjuense sp. nov. is proposed. The type strain is SS1-76 T (= KCTC 52159 T  = JCM 31375 T ).