Properties of two laccases from the Trametes hirsuta 072 multigene family: Twins with different faces

• Published in: Biochimie 2017-11, Vol.142, p.183-190
• Main Authors: Savinova, Olga S., Moiseenko, Konstantin V., Vavilova, Ekaterina A., Tyazhelova, Tatiana V., Vasina, Daria V.
• Format: Article
• Language: English
• Subjects: Constitutive and inducible isozymes; Differential expression; Isoenzymes - genetics; Laccase - genetics; Laccase multigene family; Multigene Family - genetics; Trametes - enzymology; Trametes - genetics; White-rot fungi
• ISSN: 0300-9084; 1638-6183
• DOI: 10.1016/j.biochi.2017.09.013

Utilization of laccases in biotechnology and bioremediation has created a strong demand for the characterization of new enzymes and an increase in production of known laccases. Thus, additional research into these enzymes is critically needed. In this study, we report a comparative study of the biochemical and transcriptional properties of two different laccase isozymes from Trametes hirsuta 072 – the constitutive and inducible forms. A recombinant LacC enzyme was expressed in Penicillium canescens to characterize its properties. LacC is single-purpose enzyme, unlike LacA, which can operate efficiently under a wide range of temperatures and pHs (55–70 °C and pH 3–5, respectively). LacC has a lower RedOx potential than LacA and does not oxidize substrates containing amine groups. Expression of the lacC gene was selective compared to that of the lacA gene and increased significantly in the presence of complex synthetic compounds such as dyes and xenobiotics. This study shows that laccases from the multigene families of basidiomycetes differ significantly in their properties, thus providing a complementary effect during lignin degradation. [Display omitted] •A comparative study of two T. hirsuta laccase isozymes is performed.•Constitutive and inducible isozymes markedly differ in their properties and expression.•The constitutive isozyme is more stable and less specific compared to the inducible one.•The inducible LacC can only oxidize low redox potential substrates.•The potential role of laccase isozymes in fungi is discussed.