Caveolin‐1 Secreted from Adipose Tissues and Adipocytes Functions as an Adipogenesis Enhancer

Objective Caveolin‐1 (Cav‐1) is expressed abundantly in adipose tissue and involved in many physiological processes. While Cav‐1 has been reported to be secreted in pancreatic acinar cells and LNCaP prostate cancer cells, its secretion from adipose tissue awaits investigation. Methods Cav‐1 secretio...

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Published in:Obesity (Silver Spring, Md.) Md.), 2017-11, Vol.25 (11), p.1932-1940
Main Authors: Chang, Chia‐Chu, Chen, Chen‐Yu, Wen, Hui‐Chin, Huang, Chih‐Yang, Hung, Ming‐Shiu, Lu, Hsi‐Chi, Chen, Woan‐Ling, Chang, Chung‐Ho
Format: Article
Language:English
Subjects:
Adipocytes
Adipocytes - metabolism
Adipogenesis - immunology
Adipose Tissue - metabolism
Animals
Breast cancer
Caveolin 1 - metabolism
Cell Culture Techniques
Fatty acids
Growth factors
Immunoglobulins
Insulin
Kinases
Lipids
Male
Mice
Obesity
Penicillin
Prostate cancer
Proteins
Proteomics
Rodents
Sodium
Tissues
Transfer RNA
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Summary:Objective Caveolin‐1 (Cav‐1) is expressed abundantly in adipose tissue and involved in many physiological processes. While Cav‐1 has been reported to be secreted in pancreatic acinar cells and LNCaP prostate cancer cells, its secretion from adipose tissue awaits investigation. Methods Cav‐1 secretion from 3T3‐L1 adipocytes and fat tissues from normal chow diet‐ and high‐fat diet (HFD)‐fed mice was measured. Functions and uptake of secreted Cav‐1 proteins were assessed by adding Cav‐1 back to preadipocytes and LNCaP cells. Results Cav‐1 secretion was evident in adipose tissues and were substantially promoted in HFD‐fed mice. Cav‐1 was detectable in the conditioned media of 3T3‐L1 adipocytes but not preadipocytes. Hypertrophied adipocytes induced by glucose and fatty acids secreted more Cav‐1, suggesting that hypertrophied adipocytes were responsible for enhanced Cav‐1 secretion in obese mice. Secreted Cav‐1 was taken up by preadipocytes and LNCaP cells. 3T3‐L1 preadipocytes overexpressing Cav‐1 were better differentiated, suggesting that secreted Cav‐1 may promote adipogenesis. Hypertrophied 3T3‐L1 adipocytes enhanced ERK1/2 activation, and the attenuation of ERK1/2 activity by PD98059 inhibited Cav‐1 secretion. Conclusions Cav‐1 is actively secreted from adipocytes as a putative adipogenesis enhancer. Hypertrophied adipocytes secrete Cav‐1 via ERK1/2‐dependent mechanisms to promote adipogenesis, thus establishing a vicious cycle.
ISSN:1930-7381
1930-739X
DOI:10.1002/oby.21970