Development of a photoresponsive cell culture surface: Regional enhancement of living-cell adhesion induced by local light irradiation

Using a functional polymer containing nitrospirobenzopyran residues and poly(ethylene glycol), we developed a photoresponsive cell culture surface (PRCS), at which the cell adhesion can be enhanced locally by UV light irradiation (350–400 nm, 35 mW/cm2, 5 min) even on the region where living cells e...

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Bibliographic Details
Published in:Journal of applied polymer science 2006-04, Vol.100 (1), p.495-499
Main Authors: Tada, Yuichi, Sumaru, Kimio, Kameda, Mitsuyoshi, Ohi, Katsuhide, Takagi, Toshiyuki, Kanamori, Toshiyuki, Yoshimi, Yasuo
Format: Article
Language:English
Subjects:
Applied sciences
cell adhesion
cell culture
Exact sciences and technology
light irradiation
nitrospirobenzopyran
photo-response
Physicochemistry of polymers
Polymers and radiations
Properties and characterization
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Summary:Using a functional polymer containing nitrospirobenzopyran residues and poly(ethylene glycol), we developed a photoresponsive cell culture surface (PRCS), at which the cell adhesion can be enhanced locally by UV light irradiation (350–400 nm, 35 mW/cm2, 5 min) even on the region where living cells exist. Cell adhesion was evaluated by observing BALB/3T3 fibroblasts, which had been seeded on PRCS and then irradiated with UV light regionally, after 12 h incubation and subsequent washing to remove scarcely adhering cells. As a result, it was confirmed that the number of remaining cells in irradiated region was at most 2.5 times greater than that in nonirradiated regions, suggesting the implementation of a novel scheme to manipulate living cells individually by light irradiation in a parallel and simultaneous manner. Influence of the composition of the polymer material consisting the substrate surface was also investigated systematically. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 100: 495–499, 2006
ISSN:0021-8995
1097-4628
DOI:10.1002/app.23197