Development of a photoresponsive cell culture surface: Regional enhancement of living-cell adhesion induced by local light irradiation

• Published in: Journal of applied polymer science 2006-04, Vol.100 (1), p.495-499
• Main Authors: Tada, Yuichi, Sumaru, Kimio, Kameda, Mitsuyoshi, Ohi, Katsuhide, Takagi, Toshiyuki, Kanamori, Toshiyuki, Yoshimi, Yasuo
• Format: Article
• Language: English
• Subjects: Applied sciences; cell adhesion; cell culture; Exact sciences and technology; light irradiation; nitrospirobenzopyran; photo-response; Physicochemistry of polymers; Polymers and radiations; Properties and characterization
• ISSN: 0021-8995; 1097-4628
• DOI: 10.1002/app.23197

Using a functional polymer containing nitrospirobenzopyran residues and poly(ethylene glycol), we developed a photoresponsive cell culture surface (PRCS), at which the cell adhesion can be enhanced locally by UV light irradiation (350–400 nm, 35 mW/cm2, 5 min) even on the region where living cells exist. Cell adhesion was evaluated by observing BALB/3T3 fibroblasts, which had been seeded on PRCS and then irradiated with UV light regionally, after 12 h incubation and subsequent washing to remove scarcely adhering cells. As a result, it was confirmed that the number of remaining cells in irradiated region was at most 2.5 times greater than that in nonirradiated regions, suggesting the implementation of a novel scheme to manipulate living cells individually by light irradiation in a parallel and simultaneous manner. Influence of the composition of the polymer material consisting the substrate surface was also investigated systematically. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 100: 495–499, 2006