Clinical Studies: Expression of oxidative stress-related molecules in circulating leukocytes and urine in patients with chronic viral hepatitis

Oxidative stress plays a role in pathogenesis of chronic viral hepatitis. Expression of oxidative stress-related molecules remains to be clarified. Methods: 4-hydroxy-2-nonenal (4-HNE), 4-hydroxy-2-hexenal (4-HHE), catalase, superoxide dismutase-1 (SOD-1), glutathione peroxidase-1, thioredoxin (TRX)...

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Published in:Liver international 2006-03, Vol.26 (2), p.157-165
Main Authors: Saeki, Toshiya, Ichiba, Miho, Tanabe, Naotada, Ueki, Masaru, Okamoto, Kinya, Matsunaga, Yoshiko, Hosho, Keiko, Kanbe, Takamasa, Tsuchiya, Hiroyuki, Kurimasa, Akihiro, Yamada, Sadako, Hirooka, Yasuaki, Hisatome, Ichiro, Kishimoto, Yukihiro, Suou, Takeaki, Murawaki, Yoshikazu, Kawasaki, Hironaka, Yodoi, Junji, Shiota, Goshi
Format: Article
Language:English
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Summary:Oxidative stress plays a role in pathogenesis of chronic viral hepatitis. Expression of oxidative stress-related molecules remains to be clarified. Methods: 4-hydroxy-2-nonenal (4-HNE), 4-hydroxy-2-hexenal (4-HHE), catalase, superoxide dismutase-1 (SOD-1), glutathione peroxidase-1, thioredoxin (TRX) in leukocytes and urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) were examined in 164 persons, including 130 chronic viral hepatitis patients and 34 normal individuals, by Western blot analysis and enzyme-linked immunosorbent assay, respectively. Hepatic expression of these proteins was immunohistochemically examined in 12 patients with chronic viral hepatitis, compared with three persons without liver damage. Results: The 4-HNE- beta -actin ratios in chronic viral hepatitis were significantly higher than those in normal individuals (P
ISSN:1478-3223
1478-3231
DOI:10.1111/j.1478-3231.2005.01213.x