Development of a biomimetic enzyme-linked immunosorbent assay based on molecularly imprinted polymers on paper for the detection of carbaryl

•A biomimetic enzyme-linked immunosorbent assay (BELISA) based on molecularly imprinted polymers (MIPs) with specific recognition has been developed for the detection of carbaryl.•Compared to biological antibodies, the artificial antibody based on MIPs-paper showed rapid and efficient detection for...

Full description

Saved in:
Bibliographic Details
Published in:Food chemistry 2018-02, Vol.240, p.893-897
Main Authors: Zhang, Can, Cui, Hanyu, Han, Yufeng, Yu, Fangfang, Shi, Xiaoman
Format: Article
Language:English
Subjects:
Biomimetic enzyme-linked immunosorbent assay
Biomimetics
Carbaryl
Carbaryl - analysis
Enzyme-Linked Immunosorbent Assay
Molecular Imprinting
Molecularly imprinted polymer
Polymers
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•A biomimetic enzyme-linked immunosorbent assay (BELISA) based on molecularly imprinted polymers (MIPs) with specific recognition has been developed for the detection of carbaryl.•Compared to biological antibodies, the artificial antibody based on MIPs-paper showed rapid and efficient detection for carbaryl with shorter preparation, re-usability and lower costs.•The MIPs-paper could be used to test the content of the carbaryl by BELISA, also could be used as the test paper for qualitatively inspection of carbaryl in fields. A biomimetic enzyme-linked immunosorbent assay (BELISA) which was based on molecularly imprinted polymers on paper (MIPs-paper) with specific recognition was developed. As a detector, the surface of paper was modified with γ-MAPS by hydrolytic action and anchored the MIP layer on γ-MAPS modified-paper by copolymerization to construct the artificial antibody Through a series of experimentation and verification, we successful got the MIPs-paper and established BELISA for the detection of carbaryl. The development of MIPs-paper based on BELISA was applied to detect carbaryl in real samples and validated by an enzyme-linked immunosorbent assay (ELISA) based on anti-carbaryl biological antibody. The results of these two methods (BELISA and ELISA) were well correlated (R2=0.944). The established method of MIPs-paper BELISA exhibits the advantages of low cost, higher stability and being re-generable, which can be applied as a convenient tool for the fast and efficient detection of carbaryl.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2017.07.109