Isolation and characterization of polymorphic microsatellite markers in Pagellus bogaraveo, and cross-species amplification in Sparus aurata and Dicentrarchus labrax

Several new fish species are currently being included in breeding programmes. However, as specific molecular markers have not yet been developed, this represents a commercial handicap with respect to traditional aquaculture species such as gilthead sea bream or Atlantic salmon. In the present study,...

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Bibliographic Details
Published in:Molecular ecology notes 2006-03, Vol.6 (1), p.33-35
Main Authors: PiÑera, J.A, Bernardo, D, Blanco, G, VÁzquez, E, SÁnchez, J.A
Format: Article
Language:English
Subjects:
alleles
alleles per locus
blackspot sea bream
Dicentrarchus labrax
DNA primers
genetic markers
heterozygosity
loci
Marine
microsatellite
microsatellite repeats
molecular sequence data
nucleotide sequences
Pagellus
Pagellus bogaraveo
polymerase chain reaction
polymorphic loci
Salmo salar
Sparidae
Sparus aurata
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Summary:Several new fish species are currently being included in breeding programmes. However, as specific molecular markers have not yet been developed, this represents a commercial handicap with respect to traditional aquaculture species such as gilthead sea bream or Atlantic salmon. In the present study, 12 new microsatellite loci were developed for blackspot sea bream (Pagellus bogaraveo) that show high levels of polymorphism, especially useful in parentage assignment and individual identification. In addition, cross-amplification was obtained for two important species for Spanish aquaculture, gilthead sea bream and sea bass.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2006.01130.x