Propagation of mulberry variety - S sub(54) by synseeds of axillary bud

Synseeds were produced from aseptic axillary buds of mulberry variety - S sub(54) (Morus indica L.) to investigate their in vitro and ex vitro conversion potential. The synseeds when cultured on Linsmaier and Skoog's basal medium supplemented with 6-benzyl amino purine (8.88 mu M) and 2,3,5-tri...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2006-02, Vol.84 (2), p.100220-100224
Main Authors: R., Kavyashree, M.C., Gayatri, H.M., Revanasiddaiah
Format: Article
Language:English
Subjects:
Morus indica
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Summary:Synseeds were produced from aseptic axillary buds of mulberry variety - S sub(54) (Morus indica L.) to investigate their in vitro and ex vitro conversion potential. The synseeds when cultured on Linsmaier and Skoog's basal medium supplemented with 6-benzyl amino purine (8.88 mu M) and 2,3,5-tri iodo benzoic acid (2 mu M), produced shoots after 21 days exhibiting 48.2 plus or minus 0.60 in vitro conversion response. The synseeds ex vitro conversion response was found to be 45.5 plus or minus 0.76 on soilrite mix containing half strength LS nutrients after 21 days of sowing. Further, synseeds stored at 4 degree C for 1-4 months resulted in maximum conversion response under both in vitro and ex vitro conditions, followed by development into complete plantlets without any significant loss of conversion potential. Plants regenerated from the synseeds of axillary bud were hardened, acclimatized and established in soil with varying survival frequency.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-005-9022-x