PRIMER NOTE: Isolation and characterization of microsatellite loci in Alasmidonta heterodon (Bivalvia: Unionidae)

We developed 13 species-specific microsatellite markers for the federally endangered Atlantic slope unionid Alasmidonta heterodon. Four to 18 alleles per locus were observed among 30 individuals. Observed heterozygosity throughout the loci ranged from 26.9 to 86.2% and averaged 63.6%. Estimates of i...

Full description

Saved in:
Bibliographic Details
Published in:Molecular ecology notes 2006-06, Vol.6 (2), p.365-367
Main Authors: Shaw, Kristine M, King, Tim L, Lellis, William A, Eackles, Michael S
Format: Article
Language:English
Subjects:
Alasmidonta heterodon
Bivalvia
Unionidae
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We developed 13 species-specific microsatellite markers for the federally endangered Atlantic slope unionid Alasmidonta heterodon. Four to 18 alleles per locus were observed among 30 individuals. Observed heterozygosity throughout the loci ranged from 26.9 to 86.2% and averaged 63.6%. Estimates of individual pairwise genetic distances indicated that levels of genetic diversity among loci were sufficient to produce unique multilocus genotypes for all animals surveyed. Randomization tests showed that genotypes for this collection were consistent with Hardy-Weinberg expectations, and no significant linkage disequilibrium was observed between loci. These loci therefore appear suitable for population surveys, kinship assessment and other such applications.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2005.01235.x