Recovery and quantification of a myelin oligodendrocyte glycoprotein peptide from rat plasma after protein precipitation

The recovery of high molecular weight peptides from complex biological samples is a challenging task. Herein, a reliable, cost effective and rapid methodology was developed for the recovery and quantification of a myelin oligodendrocyte glycoprotein epitope namely (LysGly)5MOG35-55, from rat plasma....

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Bibliographic Details
Published in:Analytical biochemistry 2017-12, Vol.538, p.71-73
Main Authors: Kontos, Christos, Androutsou, Maria-Eleni, Vlamis-Gardikas, Alexios, Tselios, Theodore
Format: Article
Language:English
Subjects:
Animals
Bioactive peptide
Blood Chemical Analysis - methods
Chemical Precipitation
Chromatography, High Pressure Liquid
Epitopes - blood
Epitopes - isolation & purification
HPLC
Myelin oligodendrocyte glycoprotein
Myelin-Oligodendrocyte Glycoprotein - blood
Myelin-Oligodendrocyte Glycoprotein - chemistry
Myelin-Oligodendrocyte Glycoprotein - isolation & purification
Peptides - blood
Peptides - isolation & purification
Plasma protein precipitation
Rats
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Summary:The recovery of high molecular weight peptides from complex biological samples is a challenging task. Herein, a reliable, cost effective and rapid methodology was developed for the recovery and quantification of a myelin oligodendrocyte glycoprotein epitope namely (LysGly)5MOG35-55, from rat plasma. Removal of plasma proteins before quantification of the peptide was achieved after precipitation by an acetonitrile/water/formic acid solution. Using the developed protocol, average recoveries of the peptide from plasma ranged between 83.3 and 90.3%.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2017.09.016