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Recovery and quantification of a myelin oligodendrocyte glycoprotein peptide from rat plasma after protein precipitation

The recovery of high molecular weight peptides from complex biological samples is a challenging task. Herein, a reliable, cost effective and rapid methodology was developed for the recovery and quantification of a myelin oligodendrocyte glycoprotein epitope namely (LysGly)5MOG35-55, from rat plasma....

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Published in:	Analytical biochemistry 2017-12, Vol.538, p.71-73
Main Authors:	Kontos, Christos, Androutsou, Maria-Eleni, Vlamis-Gardikas, Alexios, Tselios, Theodore
Format:	Article
Language:	English
Subjects:	Animals Bioactive peptide Blood Chemical Analysis - methods Chemical Precipitation Chromatography, High Pressure Liquid Epitopes - blood Epitopes - isolation & purification HPLC Myelin oligodendrocyte glycoprotein Myelin-Oligodendrocyte Glycoprotein - blood Myelin-Oligodendrocyte Glycoprotein - chemistry Myelin-Oligodendrocyte Glycoprotein - isolation & purification Peptides - blood Peptides - isolation & purification Plasma protein precipitation Rats
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container_title	Analytical biochemistry
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creator	Kontos, Christos Androutsou, Maria-Eleni Vlamis-Gardikas, Alexios Tselios, Theodore
description	The recovery of high molecular weight peptides from complex biological samples is a challenging task. Herein, a reliable, cost effective and rapid methodology was developed for the recovery and quantification of a myelin oligodendrocyte glycoprotein epitope namely (LysGly)5MOG35-55, from rat plasma. Removal of plasma proteins before quantification of the peptide was achieved after precipitation by an acetonitrile/water/formic acid solution. Using the developed protocol, average recoveries of the peptide from plasma ranged between 83.3 and 90.3%.
doi_str_mv	10.1016/j.ab.2017.09.016
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subjects	Animals Bioactive peptide Blood Chemical Analysis - methods Chemical Precipitation Chromatography, High Pressure Liquid Epitopes - blood Epitopes - isolation & purification HPLC Myelin oligodendrocyte glycoprotein Myelin-Oligodendrocyte Glycoprotein - blood Myelin-Oligodendrocyte Glycoprotein - chemistry Myelin-Oligodendrocyte Glycoprotein - isolation & purification Peptides - blood Peptides - isolation & purification Plasma protein precipitation Rats
title	Recovery and quantification of a myelin oligodendrocyte glycoprotein peptide from rat plasma after protein precipitation
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