Impact of cellular health conditions on the protein folding state in mammalian cells

By using in-cell NMR experiments, we have demonstrated that the protein folding state in cells is significantly influenced by the cellular health conditions. hAK1 was denatured in cells under stressful culture conditions, while it remained functional and properly folded in cells continuously supplie...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2017, Vol.53 (81), p.11245-11248
Main Authors: Inomata, Kohsuke, Kamoshida, Hajime, Ikari, Masaomi, Ito, Yutaka, Kigawa, Takanori
Format: Article
Language:English
Subjects:
Adenylate Kinase - chemistry
Adenylate Kinase - metabolism
HeLa Cells
Humans
Nuclear Magnetic Resonance, Biomolecular
Protein Folding - drug effects
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Summary:By using in-cell NMR experiments, we have demonstrated that the protein folding state in cells is significantly influenced by the cellular health conditions. hAK1 was denatured in cells under stressful culture conditions, while it remained functional and properly folded in cells continuously supplied with a fresh medium.
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc06004a