Heifers express G‐protein coupled receptor 153 in anterior pituitary gonadotrophs in stage‐dependent manner

• Published in: Animal science journal 2018-01, Vol.89 (1), p.60-71
• Main Authors: Pandey, Kiran, Kereilwe, Onalenna, Kadokawa, Hiroya
• Format: Article
• Language: English
• Subjects: Animals; Cattle; Cell Membrane - metabolism; Cells, Cultured; Conserved sequence; Cytoplasm; Cytoplasm - metabolism; Estrogens; Female; Follicle Stimulating Hormone - metabolism; Follicle-stimulating hormone; G protein-coupled receptors; Gene Expression; Gonadotrophs - cytology; Gonadotrophs - metabolism; Gonadotropin-releasing hormone; Gonadotropins; gonadotropin‐releasing hormone receptor; Immunofluorescence; lipid raft; Luteal Phase - genetics; Luteal Phase - metabolism; Luteinizing hormone; Luteinizing Hormone - metabolism; Membrane Microdomains; Microscopy; Ovulation; Ovulation - genetics; Ovulation - metabolism; Pituitary (anterior); Pituitary Gland, Anterior - cytology; Progesterone; Receptors, G-Protein-Coupled - genetics; Receptors, G-Protein-Coupled - metabolism; Receptors, LHRH - metabolism; Reproduction - physiology; Reproductive cycle; Response Elements; ruminant; Tissues
• ISSN: 1344-3941; 1740-0929
• DOI: 10.1111/asj.12920

We recently found that orphan G‐protein‐coupled receptor (GPR)153 is expressed in the anterior pituitary (AP) of heifers, leading us to speculate that GPR153 colocalizes with gonadotropin‐releasing hormone receptor (GnRHR) in the plasma membrane of gonadotrophs and is expressed at specific times of the reproductive cycle. To test this hypothesis, we examined the coexpression of GnRHR, GPR153, and either luteinizing hormone or follicle‐stimulating hormone in AP tissue and cultured AP cells by immunofluorescence microscopy. GPR153 was detected in the gonadotrophs, and was colocalized with GnRHR in the plasma membrane. GPR153 was also detected in the cytoplasm of cultured gonadotrophs. Real‐time PCR and western blot analyses found that expression was lower (P