Ancient permafrost staphylococci carry antibiotic resistance genes

Background: Permafrost preserves a variety of viable ancient microorganisms. Some of them can be cultivated after being kept at subzero temperatures for thousands or even millions of years. Objective: To cultivate bacterial strains from permafrost. Design: We isolated and cultivated two bacterial st...

Full description

Saved in:
Bibliographic Details
Published in:Microbial ecology in health and disease 2017-01, Vol.28 (1), p.1345574-8
Main Authors: Kashuba, Elena, Dmitriev, Alexey A., Kamal, Shady Mansour, Melefors, Ojar, Griva, Gennady, Römling, Ute, Ernberg, Ingemar, Kashuba, Vladimir, Brouchkov, Anatoli
Format: Article
Language:English
Subjects:
Antibiotic resistance
Antibiotics
Astrobiology
Bacteria
biofilm
Biofilms
Deoxyribonucleic acid
DNA
Drug resistance
Evolution
Gelatinase A
Gene sequencing
Genes
genome sequence
Genomes
Hypotheses
Medicin och hälsovetenskap
microbial physiology
Microbiology
Microorganisms
Miocene
Nucleotide sequence
Original
Permafrost
Ribonucleic acid
RNA
rRNA 16S
Sediments
Species
Staphylococcus
Strains (organisms)
Surveillance
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background: Permafrost preserves a variety of viable ancient microorganisms. Some of them can be cultivated after being kept at subzero temperatures for thousands or even millions of years. Objective: To cultivate bacterial strains from permafrost. Design: We isolated and cultivated two bacterial strains from permafrost that was obtained at Mammoth Mountain in Siberia and attributed to the Middle Miocene. Bacterial genomic DNA was sequenced with 40-60× coverage and high-quality contigs were assembled. The first strain was assigned to Staphylococcus warneri species (designated MMP1) and the second one to Staphylococcus hominis species (designated MMP2), based on the classification of 16S ribosomal RNA genes and genomic sequences. Results: Genomic sequence analysis revealed the close relation of the isolated ancient bacteria to the modern bacteria of this species. Moreover, several genes associated with resistance to different groups of antibiotics were found in the S. hominis MMP2 genome. Conclusions: These findings supports a hypothesis that antibiotic resistance has an ancient origin. The enrichment of cultivated bacterial communities with ancient permafrost strains is essential for the analysis of bacterial evolution and antibiotic resistance.
ISSN:0891-060X
1651-2235
1651-2235
1455-9145
DOI:10.1080/16512235.2017.1345574