Omega-3 polyunsaturated fatty acids alleviate hepatic steatosis-induced inflammation through Sirt1-mediated nuclear translocation of NF-κB p65 subunit in hepatocytes of large yellow croaker (Larmichthys crocea)

• Published in: Fish & shellfish immunology 2017-12, Vol.71, p.76-82
• Main Authors: Wang, Tianjiao, Yang, Bo, Ji, Renlei, Xu, Wei, Mai, Kangsen, Ai, Qinghui
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Fatty Acids, Omega-3 - pharmacology; Fatty Liver - etiology; Fatty Liver - veterinary; Fish Diseases - genetics; Fish Diseases - immunology; Fish Proteins - genetics; Fish Proteins - metabolism; Hepatic steatosis; Hepatocytes; Inflammation; Inflammation - genetics; Inflammation - immunology; Inflammation - veterinary; Large yellow croaker; NF-κB; Perciformes; Protein Transport; Sirt1; Sirtuin 1 - genetics; Sirtuin 1 - metabolism; Transcription Factor RelA - metabolism
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2017.09.064

Hepatic steatosis induced inflammation is becoming increasingly prevalent in farmed fish. This study was conducted to investigate the protective effects of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) against hepatic steatosis-induced inflammation and its potential molecular mechanisms in hepatocyte of large yellow croaker (Larmichthys crocea). We found that the hepatic steatosis-induced inflammation was relieved by ω-3 PUFAs, meanwhile, the Sirt1 activity and transcript expression was increased by ω-3 PUFAs. The increased Sirt1 activity can decrease the hepatic steatosis-induced inflammation. The protective effects of ω-3 PUFAs against hepatic steatosis-induced inflammation was reversed by the treatment with Sirt1 inhibitor EX-527. The nuclear translocation of nuclear transcription factor kappa-B (NF-κB) p65 was significantly decreased after ω-3 PUFAs treatments compared to the palmitic acid stimulation group. The ω-3 PUFAs induced cytoplasm translocation of NF-κB p65 was reversed by EX-527. Together, ω-3 PUFAs alleviate hepatic steatosis-induced inflammation through Sirt1-mediated nuclear translocation of NF-κB p65 subunit in hepatocytes of large yellow croaker. The present study provides important insight into the mechanisms of the protective effects of ω-3 PUFAs, providing theory bases for alleviating the hepatic steatosis induced inflammation of farmed fish, thereby offering great benefits to the aquaculture industry and fish consumers. •ω-3 PUFAs can relieve the hepatic steatosis-induced inflammation.•ω-3 PUFAs can increase Sirt1 activity and transcript expression in hepatocytes with triacylglycerol accumulation.•Sirt1 can mediate hepatic steatosis-induced inflammation via nuclear translocation of NF-κB p65.