Harnessing phage and ribosome display for antibody optimisation

Therapeutic antibodies have become a major driving force for the biopharmaceutical industry; therefore, the discovery and development of safe and efficacious antibody leads have become competitive processes. Phage and ribosome display are ideal tools for the generation of such molecules and have alr...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 2006-11, Vol.24 (11), p.523-529
Main Authors: Dufner, Patrick, Jermutus, Lutz, Minter, Ralph R.
Format: Article
Language:English
Subjects:
Antibodies - genetics
Antibodies - isolation & purification
Antibodies, Monoclonal - genetics
Antibodies, Monoclonal - isolation & purification
Antibody Specificity
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Immunoglobulin Fragments - genetics
Immunoglobulin Fragments - isolation & purification
Mutagenesis
Mutation
Peptide Library
Proteins
Ribosomes - metabolism
Technological change
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Summary:Therapeutic antibodies have become a major driving force for the biopharmaceutical industry; therefore, the discovery and development of safe and efficacious antibody leads have become competitive processes. Phage and ribosome display are ideal tools for the generation of such molecules and have already delivered an approved drug as well as a multitude of clinical candidates. Because they are capable of searching billions of antibody variants in tailored combinatorial libraries, they are particularly applicable to potency optimisation. In conjunction with targeted, random or semi-rational mutagenesis strategies, they deliver large panels of potent antibody leads. This review introduces the two technologies, compares them with respect to their use in antibody optimisation and highlights how they can be exploited for the successful and efficient generation of putative drug candidates.
ISSN:0167-7799
1879-3096
DOI:10.1016/j.tibtech.2006.09.004