Distinct roles of Cep192 and Cep152 in acentriolar MTOCs and spindle formation during mouse oocyte maturation

Mammalian oocytes lack a centriole that acts as a microtubule organization center (MTOC) in most somatic cells. During oocyte maturation, MTOCs undergo remodeling processes, including decondensation, fragmentation, and self‐organization. However, the underlying mechanisms of MTOC remodeling in mouse...

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Published in:The FASEB journal 2018-02, Vol.32 (2), p.625-638
Main Authors: Lee, In‐Won, Jo, Yu‐Jin, Jung, Seung‐Min, Wang, Hai‐Yang, Kim, Nam‐Hyung, Namgoong, Suk
Format: Article
Language:English
Subjects:
Animals
CDC2 Protein Kinase - genetics
CDC2 Protein Kinase - metabolism
CDK1
Chromosomal Proteins, Non-Histone - genetics
Chromosomal Proteins, Non-Histone - metabolism
Female
meiosis
Meiosis - physiology
Mice
Microtubule-Organizing Center - metabolism
Oocytes - cytology
Oocytes - metabolism
PCM
PLK4
Spindle Apparatus - genetics
Spindle Apparatus - metabolism
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Summary:Mammalian oocytes lack a centriole that acts as a microtubule organization center (MTOC) in most somatic cells. During oocyte maturation, MTOCs undergo remodeling processes, including decondensation, fragmentation, and self‐organization. However, the underlying mechanisms of MTOC remodeling in mouse oocytes are not well understood. We showed that two pericentriolar proteins, Cep192 and Cep152, play crucial roles during MTOC remodeling in mouse oocytes. Cep192 is present in MTOCs at all stages of oocyte maturation, and its depletion induces ablation of MTOCs, delay in spindle formation, and abnormal chromosomal alignment in spindles. In the case of Cep152, its localization on MTOCs is limited at the germinal vesicle stage and then disappears from the MTOCs after the germinal vesicle breakdown stage. Cep152 exclusion from MTOCs is involved in the fragmentation of MTOCs, and it is regulated by cyclin‐dependent kinase 1 activity. Our results demonstrate the different roles of Cep192 and Cep152 in MTOC remodeling and a novel regulatory mechanism during meiotic spindle formation in mouse oocytes.—Lee, I.‐W., Jo, Y.‐J., Jung, S.‐M., Wang, H.‐Y., Kim, N.‐H., Namgoong, S. Distinct roles of Cep192 and Cep152 in acentriolar MTOCs and spindle formation during mouse oocyte maturation. FASEB J. 32, 625–638 (2018). www.fasebj.org
ISSN:0892-6638
1530-6860
DOI:10.1096/fj.201700559RR