Characterization, multiplex conditions, and cross-species utility of tetranucleotide microsatellite loci for Blanchard's cricket frog (Acris crepitans blanchardi)

We have isolated and characterized 17 tetranucleotide microsatellite loci for Blanchard's cricket frog (Acris crepitans blanchardi), an anuran common in the central USA. Sixteen loci were organized into four multiplex amplification reactions. These loci were highly polymorphic when screened in...

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Bibliographic Details
Published in:Molecular ecology notes 2007-11, Vol.7 (6), p.1338-1341
Main Authors: BEAUCLERC, KAELA B, JOHNSON, BOB, WHITE, BRADLEY N
Format: Article
Language:English
Subjects:
Acris crepitans blanchardi
Acris crepitans crepitans
Acris gryllus
Anura
cricket frog
cross-species amplification
Gryllidae
molecular cloning
multiplex PCR
tetranucleotide microsatellite loci
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Summary:We have isolated and characterized 17 tetranucleotide microsatellite loci for Blanchard's cricket frog (Acris crepitans blanchardi), an anuran common in the central USA. Sixteen loci were organized into four multiplex amplification reactions. These loci were highly polymorphic when screened in 55 individuals from two distant populations, with 11-48 alleles per locus (average = 24.8). Observed and expected heterozygosities ranged from 0.18 to 0.97 and from 0.17 to 0.96, respectively. Nine loci were also polymorphic in Acris crepitans crepitans, with seven polymorphic in Acris gryllus. Five loci amplified in all three taxa. These loci will be useful for population- and species-level investigations of this widespread group.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2007.01874.x