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High level of CTP synthase induces formation of cytoophidia in cortical neurons and impairs corticogenesis
De novo synthesis of the nucleotide CTP is catalyzed by the essential pyrimidine biosynthesis enzyme CTP synthase (CTPs), which forms large-scale filamentous structures consisting of CTPs termed cytoophidia in prokaryotes and in eukaryotes. Recent studies have shown that cytoophidia are abundant in...
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| Published in: | Histochemistry and cell biology 2018, Vol.149 (1), p.61-73 |
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| Main Authors: | , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c372t-9fcc46b7d51f189ba1bba839d38e0b59be743c6acf44eaec81b540607f267e8b3 |
| container_end_page | 73 |
| container_issue | 1 |
| container_start_page | 61 |
| container_title | Histochemistry and cell biology |
| container_volume | 149 |
| creator | Li, Xuzhao Xie, Jiongfang Hei, Maofang Tang, Jianli Wang, Yanqing Förster, Eckart Zhao, Shanting |
| description | De novo synthesis of the nucleotide CTP is catalyzed by the essential pyrimidine biosynthesis enzyme CTP synthase (CTPs), which forms large-scale filamentous structures consisting of CTPs termed cytoophidia in prokaryotes and in eukaryotes. Recent studies have shown that cytoophidia are abundant in neuroepithelial stem cells in
Drosophila
optic lobes and that overexpression of CTPs impairs optic lobe development. Whether CTPs and cytoophidia also play a role in the development of the mammalian cortex remains elusive. Here, we show that overexpression of CTPs by in utero electroporation in the embryonic mouse brain induces formation of cytoophidia in developing cortical neurons and impairs neuronal migration. In addition, the increase of cytoophidia accelerates neuronal differentiation and inhibits neural progenitor cell proliferation by reducing their mitotic activity. Furthermore, we discovered that the cytoophidia diffused during the early G1-phase of the cell cycle. Together, our findings show, for the first time, that CTPs play a significant role in the development of the mammalian cortex. |
| doi_str_mv | 10.1007/s00418-017-1612-2 |
| format | article |
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Drosophila
optic lobes and that overexpression of CTPs impairs optic lobe development. Whether CTPs and cytoophidia also play a role in the development of the mammalian cortex remains elusive. Here, we show that overexpression of CTPs by in utero electroporation in the embryonic mouse brain induces formation of cytoophidia in developing cortical neurons and impairs neuronal migration. In addition, the increase of cytoophidia accelerates neuronal differentiation and inhibits neural progenitor cell proliferation by reducing their mitotic activity. Furthermore, we discovered that the cytoophidia diffused during the early G1-phase of the cell cycle. Together, our findings show, for the first time, that CTPs play a significant role in the development of the mammalian cortex.</description><identifier>ISSN: 0948-6143</identifier><identifier>EISSN: 1432-119X</identifier><identifier>DOI: 10.1007/s00418-017-1612-2</identifier><identifier>PMID: 28975414</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Carbon-Nitrogen Ligases - genetics ; Carbon-Nitrogen Ligases - metabolism ; Cell Biology ; Cell Cycle ; Cell migration ; Cell Proliferation ; Cerebral Cortex - cytology ; Cerebral Cortex - enzymology ; Cortex ; CTP synthase ; Cytoplasm - enzymology ; Developmental Biology ; Electroporation ; Embryos ; Female ; Mice ; Mice, Inbred Strains ; Neurogenesis - genetics ; Neurons - enzymology ; Optic lobe ; Original Paper ; Pregnancy ; Progenitor cells ; Prokaryotes ; Stem cell transplantation ; Stem cells</subject><ispartof>Histochemistry and cell biology, 2018, Vol.149 (1), p.61-73</ispartof><rights>Springer-Verlag GmbH Germany 2017</rights><rights>Histochemistry and Cell Biology is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-9fcc46b7d51f189ba1bba839d38e0b59be743c6acf44eaec81b540607f267e8b3</citedby><cites>FETCH-LOGICAL-c372t-9fcc46b7d51f189ba1bba839d38e0b59be743c6acf44eaec81b540607f267e8b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28975414$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xuzhao</creatorcontrib><creatorcontrib>Xie, Jiongfang</creatorcontrib><creatorcontrib>Hei, Maofang</creatorcontrib><creatorcontrib>Tang, Jianli</creatorcontrib><creatorcontrib>Wang, Yanqing</creatorcontrib><creatorcontrib>Förster, Eckart</creatorcontrib><creatorcontrib>Zhao, Shanting</creatorcontrib><title>High level of CTP synthase induces formation of cytoophidia in cortical neurons and impairs corticogenesis</title><title>Histochemistry and cell biology</title><addtitle>Histochem Cell Biol</addtitle><addtitle>Histochem Cell Biol</addtitle><description>De novo synthesis of the nucleotide CTP is catalyzed by the essential pyrimidine biosynthesis enzyme CTP synthase (CTPs), which forms large-scale filamentous structures consisting of CTPs termed cytoophidia in prokaryotes and in eukaryotes. Recent studies have shown that cytoophidia are abundant in neuroepithelial stem cells in
Drosophila
optic lobes and that overexpression of CTPs impairs optic lobe development. Whether CTPs and cytoophidia also play a role in the development of the mammalian cortex remains elusive. Here, we show that overexpression of CTPs by in utero electroporation in the embryonic mouse brain induces formation of cytoophidia in developing cortical neurons and impairs neuronal migration. In addition, the increase of cytoophidia accelerates neuronal differentiation and inhibits neural progenitor cell proliferation by reducing their mitotic activity. Furthermore, we discovered that the cytoophidia diffused during the early G1-phase of the cell cycle. Together, our findings show, for the first time, that CTPs play a significant role in the development of the mammalian cortex.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Carbon-Nitrogen Ligases - genetics</subject><subject>Carbon-Nitrogen Ligases - metabolism</subject><subject>Cell Biology</subject><subject>Cell Cycle</subject><subject>Cell migration</subject><subject>Cell Proliferation</subject><subject>Cerebral Cortex - cytology</subject><subject>Cerebral Cortex - enzymology</subject><subject>Cortex</subject><subject>CTP synthase</subject><subject>Cytoplasm - enzymology</subject><subject>Developmental Biology</subject><subject>Electroporation</subject><subject>Embryos</subject><subject>Female</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Neurogenesis - genetics</subject><subject>Neurons - enzymology</subject><subject>Optic lobe</subject><subject>Original Paper</subject><subject>Pregnancy</subject><subject>Progenitor cells</subject><subject>Prokaryotes</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><issn>0948-6143</issn><issn>1432-119X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kUGLFDEQhYMo7rj6A7xIwIuX1lQ6nXSOMqgrLOhhBW8hSVfPZOhOxqRbmH9vhhlFBE8FVd97VdQj5CWwt8CYelcYE9A3DFQDEnjDH5ENiJY3APr7Y7JhWvSNrJ0b8qyUA2PQac6fkhvea9UJEBtyuAu7PZ3wJ040jXT78JWWU1z2tiANcVg9FjqmPNslpHgm_GlJ6bgPQ7AVoD7lJXg70YhrTrFQGwca5qMNuVyHaYcRSyjPyZPRTgVfXOst-fbxw8P2rrn_8unz9v1941vFl0aP3gvp1NDBCL12FpyzfauHtkfmOu1QidZL60ch0KLvwXWCSaZGLhX2rr0lby6-x5x-rFgWM4ficZpsxLQWA1oopjXItqKv_0EPac2xXlcpzZWW0MpKwYXyOZWScTTHHGabTwaYOQdhLkGYGoQ5B2F41by6Oq9uxuGP4vfnK8AvQKmjuMP81-r_uv4CJGuUhA</recordid><startdate>2018</startdate><enddate>2018</enddate><creator>Li, Xuzhao</creator><creator>Xie, Jiongfang</creator><creator>Hei, Maofang</creator><creator>Tang, Jianli</creator><creator>Wang, Yanqing</creator><creator>Förster, Eckart</creator><creator>Zhao, Shanting</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>2018</creationdate><title>High level of CTP synthase induces formation of cytoophidia in cortical neurons and impairs corticogenesis</title><author>Li, Xuzhao ; Xie, Jiongfang ; Hei, Maofang ; Tang, Jianli ; Wang, Yanqing ; Förster, Eckart ; Zhao, Shanting</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-9fcc46b7d51f189ba1bba839d38e0b59be743c6acf44eaec81b540607f267e8b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Carbon-Nitrogen Ligases - genetics</topic><topic>Carbon-Nitrogen Ligases - metabolism</topic><topic>Cell Biology</topic><topic>Cell Cycle</topic><topic>Cell migration</topic><topic>Cell Proliferation</topic><topic>Cerebral Cortex - cytology</topic><topic>Cerebral Cortex - enzymology</topic><topic>Cortex</topic><topic>CTP synthase</topic><topic>Cytoplasm - enzymology</topic><topic>Developmental Biology</topic><topic>Electroporation</topic><topic>Embryos</topic><topic>Female</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Neurogenesis - genetics</topic><topic>Neurons - enzymology</topic><topic>Optic lobe</topic><topic>Original Paper</topic><topic>Pregnancy</topic><topic>Progenitor cells</topic><topic>Prokaryotes</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xuzhao</creatorcontrib><creatorcontrib>Xie, Jiongfang</creatorcontrib><creatorcontrib>Hei, Maofang</creatorcontrib><creatorcontrib>Tang, Jianli</creatorcontrib><creatorcontrib>Wang, Yanqing</creatorcontrib><creatorcontrib>Förster, Eckart</creatorcontrib><creatorcontrib>Zhao, Shanting</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Biological Science Journals</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Histochemistry and cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xuzhao</au><au>Xie, Jiongfang</au><au>Hei, Maofang</au><au>Tang, Jianli</au><au>Wang, Yanqing</au><au>Förster, Eckart</au><au>Zhao, Shanting</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High level of CTP synthase induces formation of cytoophidia in cortical neurons and impairs corticogenesis</atitle><jtitle>Histochemistry and cell biology</jtitle><stitle>Histochem Cell Biol</stitle><addtitle>Histochem Cell Biol</addtitle><date>2018</date><risdate>2018</risdate><volume>149</volume><issue>1</issue><spage>61</spage><epage>73</epage><pages>61-73</pages><issn>0948-6143</issn><eissn>1432-119X</eissn><abstract>De novo synthesis of the nucleotide CTP is catalyzed by the essential pyrimidine biosynthesis enzyme CTP synthase (CTPs), which forms large-scale filamentous structures consisting of CTPs termed cytoophidia in prokaryotes and in eukaryotes. Recent studies have shown that cytoophidia are abundant in neuroepithelial stem cells in
Drosophila
optic lobes and that overexpression of CTPs impairs optic lobe development. Whether CTPs and cytoophidia also play a role in the development of the mammalian cortex remains elusive. Here, we show that overexpression of CTPs by in utero electroporation in the embryonic mouse brain induces formation of cytoophidia in developing cortical neurons and impairs neuronal migration. In addition, the increase of cytoophidia accelerates neuronal differentiation and inhibits neural progenitor cell proliferation by reducing their mitotic activity. Furthermore, we discovered that the cytoophidia diffused during the early G1-phase of the cell cycle. Together, our findings show, for the first time, that CTPs play a significant role in the development of the mammalian cortex.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28975414</pmid><doi>10.1007/s00418-017-1612-2</doi><tpages>13</tpages></addata></record> |
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| identifier | ISSN: 0948-6143 |
| ispartof | Histochemistry and cell biology, 2018, Vol.149 (1), p.61-73 |
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| language | eng |
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| source | Springer Nature |
| subjects | Animals Biochemistry Biomedical and Life Sciences Biomedicine Carbon-Nitrogen Ligases - genetics Carbon-Nitrogen Ligases - metabolism Cell Biology Cell Cycle Cell migration Cell Proliferation Cerebral Cortex - cytology Cerebral Cortex - enzymology Cortex CTP synthase Cytoplasm - enzymology Developmental Biology Electroporation Embryos Female Mice Mice, Inbred Strains Neurogenesis - genetics Neurons - enzymology Optic lobe Original Paper Pregnancy Progenitor cells Prokaryotes Stem cell transplantation Stem cells |
| title | High level of CTP synthase induces formation of cytoophidia in cortical neurons and impairs corticogenesis |
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