Liquid biopsy in the diagnosis of HPV DNA in breast lesions

HPV DNA has never been investigated in nipple discharges (ND) and serum-derived extracellular vesicles, although its presence has been reported in ductal lavage fluids and blood specimens. We analyzed 50 ND, 22 serum-derived extracellular vesicles as well as 51 pathologic breast tissues for the pres...

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Bibliographic Details
Published in:Future microbiology 2018-02, Vol.13 (2), p.187-194
Main Authors: Carolis, Sabrina De, Pellegrini, Alice, Santini, Donatella, Ceccarelli, Claudio, De Leo, Antonio, Alessandrini, Federica, Arienti, Chiara, Pignatta, Sara, Tesei, Anna, Mantovani, Vilma, Zamagni, Claudio, Taffurelli, Mario, Sansone, Pasquale, Bonafé, Massimiliano, Cricca, Monica
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Biopsy
Breast
Breast - pathology
Breast cancer
Breast Neoplasms - pathology
Cellular biology
Deoxyribonucleic acid
DNA
DNA, Viral - secretion
Extracellular Vesicles - chemistry
Female
Genotype & phenotype
Histology
HPV DNA
Human papillomavirus
Humans
Lesions
Liquid Biopsy
Mass spectrometry
Medical diagnosis
Middle Aged
Papilloma, Intraductal - pathology
Papillomaviridae - genetics
Papillomaviridae - isolation & purification
Papillomavirus Infections - pathology
Scientific imaging
Vesicles
Womens health
Young Adult
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Description
Summary:HPV DNA has never been investigated in nipple discharges (ND) and serum-derived extracellular vesicles, although its presence has been reported in ductal lavage fluids and blood specimens. We analyzed 50 ND, 22 serum-derived extracellular vesicles as well as 51 pathologic breast tissues for the presence of 16 HPV DNA types. We show that the presence of HPV DNA in the ND is predictive of HPV DNA-positive breast lesions and that HPV DNA is more represented in intraductal papillomas. We also show the presence of HPV DNA in the serum-derived extracellular vesicles. Our data supports the use of liquid biopsy to detect HPV DNA in breast pathology.
ISSN:1746-0913
1746-0921
DOI:10.2217/fmb-2017-0145