Isolation of protoplast from aerial mycelia and fruit-body formation from regenerated mycelia in Pleurotus cornucopiae

We have developed a simple and efficient method for protoplast isolation from Pleurotus cornucopiae. Protoplasts were isolated from aerial mycelia cultured on potato dextrose agar medium without time-consuming propagation in liquid culture. Protoplast yield was significantly increased by means of a...

Full description

Saved in:
Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2006-11, Vol.70 (11), p.2751-2753
Main Authors: Mizoguchi, M.(Konan Univ., Kobe (Japan)), Kitano, S, Takahashi, W, Sugawara, F, Tanaka, O
Format: Article
Language:English
Subjects:
aerial mycelia
AISLAMIENTO
Biological and medical sciences
CELLULASE
CELULASA
DEGRADACION
DEGRADATION
ESPOROFOROS
fruit-body formation
Fundamental and applied biological sciences. Psychology
ISOLATION
ISOLEMENT
MELANGE
MEZCLADO
MICELIO
MIXING
MYCELIUM
PLEUROTUS
Pleurotus - metabolism
Pleurotus cornucopiae
protoplast
PROTOPLASTE
PROTOPLASTOS
PROTOPLASTS
Protoplasts - metabolism
REGENERACION
REGENERATION
Solanum tuberosum
SPOROPHORE
SPOROPHORES
VIBRACIONES
VIBRATION
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have developed a simple and efficient method for protoplast isolation from Pleurotus cornucopiae. Protoplasts were isolated from aerial mycelia cultured on potato dextrose agar medium without time-consuming propagation in liquid culture. Protoplast yield was significantly increased by means of a decompressing pretreatment of mycelia in enzyme solution and a subsequent enzyme reaction with vibrational mixing. The isolated protoplasts regenerated mycelia and these mycelia formed fruit-bodies without any morphological abnormalities.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.60165