MiR‐132‐3p regulates ADAMTS‐5 expression and promotes chondrogenic differentiation of rat mesenchymal stem cells

Previous study showed that miRNA aberrant expression is involved in chondrogenic differentiation. In this study, we aimed to investigate the effects of miR‐132‐3p on chondrogenic differentiation and the underlying mechanisms. First, quantitative PCR were performed to determine the level of MiR‐132‐3...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cellular biochemistry 2018-03, Vol.119 (3), p.2579-2587
Main Authors: Zhou, Xiaozhong, Luo, Dixin, Sun, Hongtao, Qi, Yong, Xu, Wangyang, Jin, Xunjie, Li, Chao, Lin, Zhousheng, Li, Guitao
Format: Article
Language:English
Subjects:
Aberration
ADAMTS‐5
Aggrecan
Biocompatibility
Biomedical materials
chondrogenic differentiation
Collagen (type II)
Differentiation
Ectopic expression
Immunofluorescence
Mesenchymal stem cells
Mesenchyme
miRNA
miR‐132‐3p
Osteoarthritis
rat mesenchymal stem cells
Sox9 protein
Stem cell transplantation
Stem cells
Target recognition
Western blotting
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Previous study showed that miRNA aberrant expression is involved in chondrogenic differentiation. In this study, we aimed to investigate the effects of miR‐132‐3p on chondrogenic differentiation and the underlying mechanisms. First, quantitative PCR were performed to determine the level of MiR‐132‐3p. Then, we used luciferase assay to examine the target of miR‐132‐3p. Proteoglycan was tested by Alcian blue staining assay. Moreover, the sex determining region Y‐box 9 (SOX9), Collagen type II alpha 1 chain (COL2A1) and Aggrecan (ACAN) levels were analyzed by quantitative PCR, immunofluorescence and Western blotting. Our results showed that MiR‐132‐3p level was reduced in rat MSCs (rMSCs) during chondrogenic differentiation. Ectopic expression of miR‐132‐3p induced proteoglycan accumulation and the increase of ACAN, SOX9 and COL2A1 expression, which were involved in inducing chondrogenic differentiation of rMSCs. More importantly, ADAMTS‐5 was identified as the target of MiR‐132‐3p. Knockdown of ADAMTS‐5 increased proteoglycan level, but reduced the SOX9, ACAN, and COL2A1 levels during chondrogenic differentiation of rMSCs. Taken together, our results revels that MiR‐132‐3p promotes rMSCs chondrogenic differentiation, possibly mediated by targeting ADAMTS‐5, which provided new perspective on the chondrogenic differentiation and pathology of osteoarthritis. Elevated miR‐132‐3p increased the levels of SOX9, ACAN and COL2A1 in the process of rMSCs’ chondrogenic differentiation.
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.26421