Reduced susceptibility to DDT in field populations of Anopheles quadriannulatus and Anopheles arabiensis in Malawi: evidence for larval selection

• Published in: Medical and veterinary entomology 2008-09, Vol.22 (3), p.258-263
• Main Authors: MZILAHOWA, T, BALL, A.J, BASS, C, MORGAN, J.C, NYONI, B, STEEN, K, DONNELLY, M.J, WILDING, C.S
• Format: Article
• Language: English
• Subjects: adult insects; agriculture; alleles; Animals; Anopheles - drug effects; Anopheles - genetics; Anopheles arabiensis; Anopheles gambiae; Anopheles gambiae s.l; Anopheles quadriannulatus; bioassays; breeding sites; Culicidae; Databases, Genetic; DDT; DDT (pesticide); DDT - pharmacology; deltamethrin; Diptera; Female; gender differences; genotype; Insecticide Resistance; Insecticides - pharmacology; kdr resistance alleles; Larva - drug effects; Larva - genetics; larvae; lethal time 50; malathion; Malathion - pharmacology; Malawi; Male; natural selection; Nitriles - pharmacology; permethrin; Permethrin - pharmacology; pest identification; Pyrethrins - pharmacology; selection; toxicity
• ISSN: 0269-283X; 1365-2915
• DOI: 10.1111/j.1365-2915.2008.00736.x

Bioassays for insecticide resistance in adult mosquitoes were conducted on samples of Anopheles gambiae Giles s.l. (Diptera: Culicidae) species collected as larvae from breeding sites in the lower Shire Valley, Malawi. The results indicate full susceptibility to permethrin, deltamethrin and malathion, but reduced susceptibility to DDT in one sample from Thom (LT₅₀ of 8.39 min for females and 25.09 min for males). Polymerase chain reaction-based species identification of the mosquitoes assayed revealed a mixture of Anopheles arabiensis Patton and Anopheles quadriannulatus (Theobold). The LT₅₀ did not differ significantly between species. Genotyping of the L1014F and L1014S kdr alleles showed all mosquito specimens to be homozygous wild type; thus the reduced susceptibility detected is not attributable to target site insensitivity and instead is likely to be metabolic in nature. Anopheles quadriannulatus is characteristically zoophagic and exophilic. Indeed, of 82 Anopheles collected through knockdown collections within dwellings, only one was An. quadriannulatus and the rest were An. arabiensis. They are unlikely, therefore, to have been exposed to selection pressure arising from insecticide-treated net usage or to DDT indoor residual spraying. Therefore, it is suggested that this example of reduced susceptibility to DDT in An. quadriannulatus reflects selection in the larval stages.