Binding of phosphatidylinositol-3,4,5-triphosphate to 45-kDa Sec14-like protein from the rat olfactory epithelium in liposomes

The binding of phosphatidylinositol-3,4,5-triphosphate to a protein with molecular mass of 45 kDa from rat olfactory epithelium (p45) was investigated using a model membrane system. Liposomes containing a mixture of phospholipids (phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinosit...

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Bibliographic Details
Published in:Russian journal of bioorganic chemistry 2006-05, Vol.32 (3), p.301-302
Main Authors: Il’nitskaya, E. V., Shamborant, O. G., Radchenko, V. V., Shuvaeva, T. M., Lipkin, V. M.
Format: Article
Language:English
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Summary:The binding of phosphatidylinositol-3,4,5-triphosphate to a protein with molecular mass of 45 kDa from rat olfactory epithelium (p45) was investigated using a model membrane system. Liposomes containing a mixture of phospholipids (phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositol-3,4,5-triphosphate) were used in the study. The binding of the protein to liposomes caused by its interaction with phosphatidylinositol-3,4,5-triphosphate was confirmed by cosedimentation and immunoblotting with chemiluminescent detection using monoclonal antibodies to the native protein p45.
ISSN:1068-1620
1573-9163
1608-330X
DOI:10.1134/S1068162006030137