Labelling Proteins with Carbon Nanodots

We present efficient labelling of several proteins with orange‐emissive carbon dots. N‐Hydroxysuccinimide was used to activate the carboxyl groups of carbon dots, which subsequently reacted with the lysine groups present on the protein. Labelling was confirmed by UV absorption spectroscopy, PAGE and...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2017-12, Vol.18 (24), p.2385-2389
Main Authors: Rao, Chethana, Khan, Syamantak, Verma, Navneet C., Nandi, Chayan Kanti
Format: Article
Language:English
Subjects:
Absorption spectroscopy
Carbon
Carbon dots
Fluctuations
Fluorescence
Fluorescence spectroscopy
Labeling
Lysine
nanoparticles
protein labeling
Proteins
Spectroscopy
Spectrum analysis
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Summary:We present efficient labelling of several proteins with orange‐emissive carbon dots. N‐Hydroxysuccinimide was used to activate the carboxyl groups of carbon dots, which subsequently reacted with the lysine groups present on the protein. Labelling was confirmed by UV absorption spectroscopy, PAGE and fluorescence correlation spectroscopy. Protein‐conjugated carbon dots showed an enhancement in fluorescence lifetime and intensity owing to reduced intramolecular dynamic fluctuations. Single‐molecule fluorescence measurements showed reduced fluorescence fluctuations and higher photon budget after protein tagging. Our study opens up opportunities to use carbon dots as highly precise biolabelling probes. Reading the label: The precise labelling of carbon dots with several proteins specifically through lysine residues by using the N‐hydroxysuccinimide‐ester reaction is presented. The optical properties are improved upon protein conjugation. This strategy can be used with any carbon dots with carboxyl functional groups to label any protein containing lysine residues.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201700440