High-Throughput Low-Background G‑Quadruplex Aptamer Chemiluminescence Assay for Ochratoxin A Using a Single Photonic Crystal Microsphere

We reported a novel hemin-G-quadruplex aptamer chemiluminescence assay platform for ochratoxin A (OTA) using the single silica photonic crystal microsphere (SPCM). The oligonucleotide A sequence containing aptamer sequences of hemin and OTA is immobilized on the surface of SPCM. The other oligonucle...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2017-11, Vol.89 (21), p.11862-11868
Main Authors: Shen, Peng, Li, Wei, Liu, Yan, Ding, Zhi, Deng, Yang, Zhu, Xuerui, Jin, Yanhao, Li, Yichen, Li, Jianlin, Zheng, Tiesong
Format: Article
Language:English
Subjects:
Analytical chemistry
Aptamers
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - metabolism
Assaying
Background noise
Biological activity
Biosensing Techniques - methods
Chains
Chemiluminescence
Chemistry
Edible Grain - chemistry
Food Contamination - analysis
G-Quadruplexes
Hemin
Hemin - chemistry
High-throughput screening
Hybridization
Luminescence
Luminescent Measurements
Microspheres
Models, Molecular
Molecular Conformation
Ochratoxin A
Ochratoxins - analysis
Ochratoxins - chemistry
Ochratoxins - metabolism
Oligonucleotides
Peptides
Photonic crystals
Photons
Silica
Silicon dioxide
Surface Properties
Toxins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We reported a novel hemin-G-quadruplex aptamer chemiluminescence assay platform for ochratoxin A (OTA) using the single silica photonic crystal microsphere (SPCM). The oligonucleotide A sequence containing aptamer sequences of hemin and OTA is immobilized on the surface of SPCM. The other oligonucleotide B sequence containing a partially complementary sequence with one part OTA aptamer and one part hemin aptamer is used as a blocking chain. The hybridization between chain A and chain B will be influenced by the presence or absence of OTA in the system, which will affect the bioactivity of DNAzyme. Thus, the chemiluminescence signal depends on the concentration of OTA in the samples. In the single particle assay platform, the signal/noise is remarkably enhanced, and the background signal can be ignored by separating hemin from the surface of SPCM. The limit of detection of the new method reaches to the pg/mL scale, and the linear detection range is 4 orders of magnitude for OTA. The new assay platform can provide a sensitive, cost-efficient, simple, and high-throughput screening for OTA.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.7b03592