Prominent IgE-binding and cytokine-inducing capacities of a newly cloned N-terminal region of Der f 14, an apolipophorin-like house dust mite allergen

• Published in: Journal of biochemistry (Tokyo) 2018-01, Vol.163 (1), p.51-60
• Main Authors: ElRamlawy, Kareem Gamal, Fujimura, Takashi, Aki, Tsunehiro, Okada, Akiyasu, Suzuki, Takayuki, Abe, Takuya, Hayashi, Takaharu, Epton, Michael J, Thomas, Wayne R, Rafeet, Inas Hussein, Al-Azhary, Diaa Beshr, Ono, Kazuhisa, Kawamoto, Seiji
• Format: Article
• Language: English
• ISSN: 0021-924X; 1756-2651
• DOI: 10.1093/jb/mvx060

We previously characterized a 177-kDa allergen, M-177, from Dermatophagoides farinae. Thereafter, a counterpart to M-177 for Euroglyphus maynei was cloned as Eur m 14, and its sequence revealed that two environmental allergens, Mag 1 and Mag 3, are digested fragments of M-177. The aims of this study were to clone the cDNA of Der f 14 corresponding to M-177 and to elucidate the allergenic capacities of the N-terminal fragment of Der f 14 (Der f 14-N). Recombinant allergens were produced as trigger-factor-fused proteins in Escherichia coli. Der f 14-N showed the highest IgE-binding frequency among Der f 14-derived fragments in patients allergic to house dust mite by enzyme-linked immunosorbent assay. Der f 14-N showed the highest capacity to induce cell proliferation in murine lymphocyte and human peripheral mononuclear cells among Der f 14-derived fragments. Der f 14-N induced IL-13, IFN-γ and IL-17 production more than Der f 1 and Der f 2 in mouse, and induced IL-5 and IFN-γ production at levels comparable to those of Der f 1 and Der f 2 in some patients. The high prevalence of IgE binding to the Der f 14-N indicates that it could be an important mite allergen.