Analysis of denaturation of bovine IgG by heat and high pressure using an optical biosensor

The therapeutic potential of bovine colostrum is generally attributed to its high content of immunoglobulin G (IgG), the physiological activity of which is vulnerable to potentially denaturing conditions during processing. The influence of both thermal and high-pressure treatments on native IgG was...

Full description

Saved in:
Bibliographic Details
Published in:International dairy journal 2008-04, Vol.18 (4), p.359-366
Main Authors: Indyk, Harvey E., Williams, Jacob W., Patel, Hasmukh A.
Format: Article
Language:English
Subjects:
animal proteins
antibody detection
Biological and medical sciences
Biosensor
Biosensors
Biotechnology
cattle
Colostrum
cow colostrum
Denaturation
epitopes
Fc domain
Food industries
Fundamental and applied biological sciences. Psychology
heat stability
heat treatment
high pressure treatment
Immunoassay
immunoassays
Immunoglobulin G
Methods. Procedures. Technologies
Milk and cheese industries. Ice creams
optical biosensors
optical equipment
protein G
protein subunits
Surface plasmon resonance
Various methods and equipments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The therapeutic potential of bovine colostrum is generally attributed to its high content of immunoglobulin G (IgG), the physiological activity of which is vulnerable to potentially denaturing conditions during processing. The influence of both thermal and high-pressure treatments on native IgG was evaluated using an optical biosensor immunoassay technique that exploited the simultaneous detection of bovine IgG by both anti-bovine IgG antibody and protein G. Whereas anti-bovine IgG revealed integrity of globally distributed native conformational epitopes, protein G specifically revealed structural stability of the Fc domain. The results confirmed comparable regional stabilities to heat and hydrostatic pressure when isolated IgG was studied, whereas a modest domain differentiation was apparent in colostrum, which may be attributed to the protective effects of unidentified colostral components.
ISSN:0958-6946
1879-0143
DOI:10.1016/j.idairyj.2007.10.004