Cloning and Expression Analysis of HbPR-1b and HbPR-3 in Hevea brasiliensis During Inoculation with Rigidoporus microporus

Pathogenesis-related (PR) proteins are dramatically accumulated after pathogen infection. Innate defense response through increasing PR-proteins is important for rubber rootstock selection that is tolerant to the white root disease caused by Rigidoporus microporus. This study was aimed to investigat...

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Published in:Pakistan journal of biological sciences 2017, Vol.20 (5), p.233-243
Main Authors: Woraathasin, Natthakorn, Nakkanong, Korakot, Nualsri, Charassri
Format: Article
Language:English
Subjects:
Cloning, Molecular - methods
Evolution, Molecular
Fungi - pathogenicity
Gene Expression Regulation, Plant
Hevea - genetics
Hevea - growth & development
Hevea - microbiology
Host-Pathogen Interactions
Phylogeny
Plant Diseases - genetics
Plant Diseases - microbiology
Plant Leaves - genetics
Plant Leaves - microbiology
Plant Proteins - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Plant - genetics
RNA, Plant - metabolism
Seedlings - genetics
Seedlings - microbiology
Time Factors
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Summary:Pathogenesis-related (PR) proteins are dramatically accumulated after pathogen infection. Innate defense response through increasing PR-proteins is important for rubber rootstock selection that is tolerant to the white root disease caused by Rigidoporus microporus. This study was aimed to investigate the expression levels of PR-1 and PR-3 genes in tolerant (PB5/51) and susceptible (BPM24 and RRIM600) rubber clones after R. microporus infection. The mRNA of HbPR-1b and HbPR-3 was isolated and characterized from rubber leaves. Gene expression levels of HbPR-1b and HbPR-3 were compared among three rubber clones (PB5/51, BPM24 and RRIM600) after R. microporus infection at 0, 12, 24, 48, 72 and 96 h using quantitative real-time PCR. The relative transcript abundances between inoculated and control plants were compared using the means of gene expression between time points and by Tukey's HSD test. A probability value (p
ISSN:1028-8880
DOI:10.3923/pjbs.2017.233.243