Microarray analysis of acaricide-inducible gene expression in the southern cattle tick, Rhipicephalus (Boophilus) microplus

Acaricide-inducible differential gene expression was studied in larvae of Rhipicephalus (Boophilus) microplus using a microarray-based approach. The acaricides used were: coumaphos, permethrin, ivermectin, and amitraz. The microarrays contained over 13 000 probes, having been derived from a previous...

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Bibliographic Details
Published in:Insect molecular biology 2008-12, Vol.17 (6), p.597-606
Main Authors: Saldivar, L, Guerrero, F. D, Miller, R. J, Bendele, K. G, Gondro, C, Brayton, K. A
Format: Article
Language:English
Subjects:
acaricide resistance
amitraz
Animals
Boophilus microplus
coumaphos
Coumaphos - toxicity
detoxification enzymes
DNA Primers - genetics
gene expression
gene expression regulation
Gene Expression Regulation - drug effects
genes
glutathione transferase
ivermectin
Ivermectin - toxicity
Ixodidae
larvae
legumain
microarray technology
microarrays
Oligonucleotide Array Sequence Analysis
organophosphates
permethrin
Permethrin - toxicity
Pesticides - toxicity
proteins
Reverse Transcriptase Polymerase Chain Reaction
Rhipicephalus
Rhipicephalus (Boophilus) microplus
Ticks - genetics
Ticks - metabolism
Toluidines - toxicity
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Summary:Acaricide-inducible differential gene expression was studied in larvae of Rhipicephalus (Boophilus) microplus using a microarray-based approach. The acaricides used were: coumaphos, permethrin, ivermectin, and amitraz. The microarrays contained over 13 000 probes, having been derived from a previously described R. microplus gene index (BmiGI Version 2; Wang et al., 2007). Relative quantitative reverse transcriptase-PCR, real time PCR, and serial analysis of gene expression data was used to verify microarray data. Among the differentially expressed genes with informative annotation were legumain, glutathione S-transferase, and a putative salivary gland-associated protein.
ISSN:1365-2583
0962-1075
1365-2583
DOI:10.1111/j.1365-2583.2008.00831.x