Acute liver damage induced by 2-nitropropane in rats: Effect of diphenyl diselenide on antioxidant defenses

• Published in: Chemico-biological interactions 2006-03, Vol.160 (2), p.99-107
• Main Authors: Borges, Lysandro P., Nogueira, Cristina Wayne, Panatieri, Rodrigo B., Rocha, João Batista Teixeira, Zeni, Gilson
• Format: Article
• Language: English
• Subjects: 2-Nitropropane; Acute Disease; Animals; Antioxidant; Antioxidants - pharmacology; Ascorbic acid; Benzene Derivatives - pharmacology; Catalase; Chemical and Drug Induced Liver Injury - metabolism; Chemical and Drug Induced Liver Injury - pathology; Chemical and Drug Induced Liver Injury - prevention & control; Disease Models, Animal; Dose-Response Relationship, Drug; Drug Interactions; Enzymes - metabolism; Free Radical Scavengers; Hepatic damage; Kidney - drug effects; Kidney - metabolism; Lipid Peroxidation - drug effects; Liver - drug effects; Liver - metabolism; Liver - pathology; Liver Function Tests; Male; Nitroparaffins - toxicity; Organoselenium; Organoselenium Compounds - pharmacology; Propane - analogs & derivatives; Propane - toxicity; Rats; Rats, Wistar; Reactive Oxygen Species - metabolism; SOD; Solvents - toxicity
• ISSN: 0009-2797; 1872-7786
• DOI: 10.1016/j.cbi.2005.12.010

The effect of post-treatment with diphenyl diselenide on liver damage induced by 2-nitropropane (2-NP) was examined in male rats. Rats were pre-treated with a single dose of 2-NP (100 mg/kg body weight dissolved in canola oil). Afterward, the animals were post-treated with a dose of diphenyl diselenide (10, 50 or 100 μmol/kg). The parameters that indicate tissue damage such as liver histopathology, plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), γ-glutamyl transferase (GGT), urea and creatinine were determined. Since the liver damage induced by 2-NP is related to oxidative damage, lipid peroxidation, superoxide dismutase (SOD), catalase (CAT) and ascorbic acid level were also evaluated. Diphenyl diselenide (50 and 100 μmol/kg) effectively restored the increase of ALT and AST activities and urea level when compared to the 2-NP group. At the higher dose, diphenyl diselenide decreased GGT activity. Treatment with diphenyl diselenide, at all doses, effectively ameliorated the increase of hepatic and renal lipid peroxidation when compared to 2-NP group. 2-NP reduced CAT activity and neither alter SOD activity nor ascorbic acid level. This study points out the involvement of CAT activity in 2-NP-induced acute liver damage and suggests that the post-treatment with diphenyl diselenide was effective in restoring the hepatic damage induced by 2-NP.