Detection of nodavirus in seawater from rearing facilities for Atlantic halibut Hippoglossus hippoglossus larvae

We used (1) ultracentrifugation followed by RT-PCR and (2) real-time RT-PCR to detect and quantify nodaviruses in seawater in which Atlantic halibut Hippoglossus hippoglossus larvae/fry had been held at rearing facilities. Evaluated against in vitro propagated viruses, the viral concentration corres...

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Bibliographic Details
Published in:Diseases of aquatic organisms 2006-01, Vol.73 (3), p.201-205
Main Authors: Nerland, Audun H, Skaar, Cecilie, Eriksen, Tove Boge, Bleie, Hogne
Format: Article
Language:English
Subjects:
Hippoglossus hippoglossus
Marine
Nodavirus
Nodaviruses
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Summary:We used (1) ultracentrifugation followed by RT-PCR and (2) real-time RT-PCR to detect and quantify nodaviruses in seawater in which Atlantic halibut Hippoglossus hippoglossus larvae/fry had been held at rearing facilities. Evaluated against in vitro propagated viruses, the viral concentration corresponded to 1.6 x 10 super(4) TCID sub(50) (50% tissue culture infectious dose) ml super(-1). Evaluated against in vitro transcribed RNA, the concentration was estimated at 2 x 10 super(7) virus particles ml super(-1) seawater.
ISSN:0177-5103
1616-1580