Dynamics of surface neurotransmitter receptors and transporters in glial cells: Single molecule insights

• Published in: Cell calcium (Edinburgh) 2017-11, Vol.67, p.46-52
• Main Authors: Ciappelloni, Silvia, Murphy-Royal, Ciaran, Dupuis, Julien P., Oliet, Stéphane H.R., Groc, Laurent
• Format: Article
• Language: English
• Subjects: Animals; Astrocyte; Astrocytes - cytology; Astrocytes - metabolism; Brain - cytology; Brain - metabolism; Calcium Signaling; Cell Membrane - metabolism; Gene Expression Regulation; Glutamate receptor; Glutamate transporters; Glutamic Acid - metabolism; Humans; Ion Channels - genetics; Ion Channels - metabolism; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; Neuroglia - cytology; Neuroglia - metabolism; Neurons - cytology; Neurons - metabolism; Organ Specificity; Protein Transport; Receptors, Neurotransmitter - genetics; Receptors, Neurotransmitter - metabolism; Single Molecule Imaging; Single nanoparticle imaging; Synapses - physiology
• ISSN: 0143-4160; 1532-1991
• DOI: 10.1016/j.ceca.2017.08.009

[Display omitted] •Principle of single nanoparticle tracking on live glial cells.•Single molecule imaging revealed high surface dynamics of proteins onto astrocytes.•Neurotransmitter receptor and transporter surface dynamics is regulated onto astrocytes.•Neurotransmitter transporter surface dynamics on astrocytes shapes glutamate transmission. The surface dynamics of neurotransmitter receptors and transporters, as well as ion channels, has been well-documented in neurons, revealing complex molecular behaviour and key physiological functions. However, our understanding of the membrane trafficking and dynamics of the signalling molecules located at the plasma membrane of glial cells is still in its infancy. Yet, recent breakthroughs in the field of glial cells have been obtained using combination of superresolution microscopy, single molecule imaging, and electrophysiological recordings. Here, we review our current knowledge on the surface dynamics of neurotransmitter receptors, transporters and ion channels, in glial cells. It has emerged that the brain cell network activity, synaptic activity, and calcium signalling, regulate the surface distribution and dynamics of these molecules. Remarkably, the dynamics of a given neurotransmitter receptor/transporter at the plasma membrane of a glial cell or neuron is unique, revealing the existence of cell-type specific regulatory pathways. Thus, investigating the dynamics of signalling proteins at the surface of glial cells will likely shed new light on our understanding of glial cell physiology and pathology.