Comprehensive assessment of electrospun scaffolds hemocompatibility

• Published in: Materials Science & Engineering C 2018-01, Vol.82, p.330-335
• Main Authors: Horakova, Jana, Mikes, Petr, Saman, Ales, Svarcova, Tereza, Jencova, Vera, Suchy, Tomas, Heczkova, Bohdana, Jakubkova, Sarka, Jirousova, Jaroslava, Prochazkova, Renata
• Format: Article
• Language: English
• Subjects: Activation; Biocompatible Materials - chemical synthesis; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Biodegradability; Biodegradation; Blood Cells - cytology; Blood Cells - drug effects; Blood Cells - metabolism; Blood compatibility; Chemical composition; Coagulation; Collagen; Collagen - chemistry; Copolymer of polylactide and polycaprolactone; Dilution; Effects; Electrospinning; Fibrous materials; Fibrous scaffolds; Hemolysis; Hemolysis - drug effects; Humans; Incubation; Materials science; Microscopy, Electron, Scanning; Molecular weight; Morphology; Partial Thromboplastin Time; Polycaprolactone; Polyester resins; Polyesters; Polyesters - chemistry; Polylactic acid; Polymers - chemical synthesis; Polymers - chemistry; Prothrombin; Prothrombin Time; Scaffolds; Thromboplastin
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2017.05.011

Biodegradable polyesters, namely polycaprolactone (PCL) and copolymer of polylactide and polycaprolactone (PLCL) were electrospun into various fibrous structures and their hemocompatibility was evaluated in vitro. Firstly, hemolytic effect was evaluated upon incubation with diluted whole blood. The results showed that the degree of hemolysis depended on chemical composition and fibrous morphology. Electrospun polycaprolactone induced slight degree of hemolysis depending on its molecular weight and fibrous morphology; copolymer PLCL did not cause detectable hemolysis. The influence of coagulation pathways was examined by measurement of coagulation times. It was showed that intrinsic coagulation pathway assessed by activated partial thromboplastin time (APTT) was moderately accelerated after incubation with PCL and prolonged after incubation with copolymer PLCL. Extrinsic activation of coagulation tested by prothrombin time (PT) was slightly accelerated after incubation with all tested electrospun samples. Thrombogenicity assessment of fibrous samples revealed high thrombogenic properties of fibrous materials that was comparable to high degree of collagen thrombogenicity. The level of platelet activation was dependent on chemical composition and surface morphology of tested materials. [Display omitted] •The interaction of electrospun scaffold with blood depends on fibrous morphology and chemical composition of the scaffold.•Fibrous samples caused high activation of platelets compared to flat foils made from the same polymers.•Thrombogenicity of electrospun biodegradable mats is comparable to collagen samples.