Dual-Signal Microbial Biosensor for the Detection of Dopamine without Inference from Other Catecholamine Neurotransmitters

Dopamine, one of catecholamine neurotransmitters, plays an important role in many brain functions and behavioral responses. In this study, we developed a novel dual-signal whole-cell biosensor for the detection of dopamine through the generation of red fluorescent proteins and 6-decarboxylated betax...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2017-11, Vol.89 (21), p.11178-11182
Main Authors: Lin, Yu-Kuan, Yeh, Yi-Chun
Format: Article
Language:English
Subjects:
Base Sequence
Betaxanthins - metabolism
Biosensing Techniques - methods
Biosensors
Brain
Catecholamine
Catecholamines
Chemistry
Dihydroxyphenylalanine
Dopamine
Dopamine - analysis
Dopamine - metabolism
Epinephrine
Escherichia coli - genetics
Fluorescence
Genetic Engineering
Levodopa
Limit of Detection
Luminescent Proteins - genetics
Luminescent Proteins - metabolism
Microorganisms
Neurotransmitters
Norepinephrine
Pigments
Promoter Regions, Genetic - genetics
Proteins
Red Fluorescent Protein
Roles
Spectrophotometry, Ultraviolet
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Summary:Dopamine, one of catecholamine neurotransmitters, plays an important role in many brain functions and behavioral responses. In this study, we developed a novel dual-signal whole-cell biosensor for the detection of dopamine through the generation of red fluorescent proteins and 6-decarboxylated betaxanthin pigments. The proposed system responses specifically to dopamine with a detection limit of 1.43 μM. Furthermore, a combination of dual output signals makes it possible to reduce the interference from other catecholamine neurotransmitters, including L-DOPA, epinephrine, and norepinephrine.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.7b02498