Autoradiolysis of the 2-deoxy-2-[18F]fluoro-D-glucose radiopharmaceutical

• Published in: Journal of radioanalytical and nuclear chemistry 2005-06, Vol.264 (3), p.595-602
• Main Authors: Búriová, E., Macášek, F., Melichar, F., Kropáček, M., Procházka, L.
• Format: Article
• Language: English
• ISSN: 0236-5731; 1588-2780
• DOI: 10.1007/s10967-005-0759-9

To control virtually the toxic compounds and to improve quality control of the solution of 2-deoxy-2-[ super(18)F]fluoro-d-glucose (2-[ super(18)F]FDG), the products of its autoradiolysis were analyzed by high-performance liquid chromatography with electrospray mass spectrometric and radiometric detectors (HPLC/MS/RAD), thin layer chromatography on TLC silica plate and HPTLC on amino modified silica plate. Except Kryptofix super( registered )2.2.2, glucose and fluoride anion, no by-products and impurities were observed by LC/MS analysis of fresh 2-[ super(18)F]FDG samples. The analysis performed in the time interval of 6 to 48 hours after the end of 2-[ super(18)F]FDG synthesis indicated that the activity of the autoradiolysis products separated by HPLC did not exceed 1.3%. As the main autoradiolysis products of 3.3 super( .) 10 super(-5) to 4.4 super( .) 10 super(-5)M 2-[ super(18)F]FDG solution of original specific activity 0.5-1.5 GBq super( .) cm super(-3) were established: arabinose - 2.8 mu M (G= 0.07/100 eV), gluconic and glucuronic acids 1.8-0.5 mu M (G =0.01-0.05/100 eV), arabinose and araburonic acids occurred under 0.5 mu M concentration at residual glucose contents about 0.14 mM. Radiation chemical yields of active products were calculated from molar activity of 2-[ super(18)F]FDG and the percentage of their activity: 0.5% radiochemical yield of 2-[ super(18)F]fluoroglucuronic acid corresponds to the G = 0.004/100 eV and 0.3% yield of 2-[ super(18)F]fluorogluconic acid issues G = 0.003/100 eV.