The exploration of endocytic mechanisms of PLA-PEG nanoparticles prepared by coaxialtri-capillary electrospray-template removal method

[Display omitted] •The nano-sized PLA-PEG particles were efficiently prepared by using coaxial tri-capillary electrospray-template removal method.•Caveolae-mediated endocytosis was one of endocytosis pathways in A549 cells for PLA-PEG nanoparticles.•Clathrin mediated endocytosis was not involved in...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2018-01, Vol.161, p.10-17
Main Authors: Chen, Jiaming, Cao, Lihua, Cui, Yuecheng, Tu, Kehua, Wang, Hongjun, Wang, Li-Qun
Format: Article
Language:English
Subjects:
Cellular uptake mechanisms
Drug release
Electrospray
Intracellular distribution
PLA-PEG nanoparticles
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Summary:[Display omitted] •The nano-sized PLA-PEG particles were efficiently prepared by using coaxial tri-capillary electrospray-template removal method.•Caveolae-mediated endocytosis was one of endocytosis pathways in A549 cells for PLA-PEG nanoparticles.•Clathrin mediated endocytosis was not involved in the endocytosis process.•The endocytosed PLA-PEG nanoparticles enriched in the head of A549 cells.•A small amount of PLA-PEG nanoparticles was transported into lysosome after 24h incubation. The nano-sized poly(lactic acid)-poly(ethylene glycol) (PLA-PEG) particles with core-shell structure were efficiently prepared by using coaxial tri-capillary electrospray-template removal method. The cellular uptake mechanism, intracellular distribution and exocytosis in A549 cell model of electrosprayed PLA-PEG nanoparticles were systemically studied. The drug release behavior of electrosprayed PLA-PEG nanoparticles were also investigated. Our results showed that PLA-PEG nanoparticles can be endocytosed quickly by A549 cells. The cellular uptake of PLA-PEG nanoparticles was an energy dependent endocytosis process. Caveolae-mediated endocytosis was only one of endocytosis pathways in A549 cells for PLA-PEG nanoparticles, while clathrin mediated endocytosis was not involved in the endocytosis process. The endocytosed PLA-PEG nanoparticles enriched in the head of A549 cells and only a small amount of them was transported into lysosome after 24h incubation. These findings provided insights into the application of electrosprayed PLA-PEG nanoparticles in nano drug delivery field.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2017.09.062