Identification of ESTs differentially expressed in green and albino mutant bamboo (Bambusa edulis) by suppressive subtractive hybridization (SSH) and microarray analysis

To gain a better understanding of gene expression in bamboo (Bambusa edulis Murno), we have used a combination of suppressive subtractive hybridization (SSH), microarray hybridization analysis, sequencing, and bioinformatics to identify bamboo genes differentially expressed in a bamboo albino mutant...

Full description

Saved in:
Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2006-08, Vol.86 (2), p.169-175
Main Authors: Lin, C.S, Lai, Y.H, Sun, C.W, Liu, N.T, Tsay, H.S, Chang, W.C, Chen, J.J.W
Format: Article
Language:English
Subjects:
Bambusa
Biological and medical sciences
Biotechnology
chloroplast DNA
complementary DNA
Eukaryotic cell cultures
expressed sequence tags
Fundamental and applied biological sciences. Psychology
gene expression
genetic variation
hybridization
Methods. Procedures. Technologies
microarray technology
Miscellaneous
molecular sequence data
mutants
nucleotide sequences
photosynthesis
Phyllostachys edulis
plant breeding
Plant cells and fungal cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To gain a better understanding of gene expression in bamboo (Bambusa edulis Murno), we have used a combination of suppressive subtractive hybridization (SSH), microarray hybridization analysis, sequencing, and bioinformatics to identify bamboo genes differentially expressed in a bamboo albino mutant. Ten expressed sequence tags (ESTs) were found to be differentially expressed; these were isolated and sequenced. RT-PCR analysis of these ESTs supported the results of the microarray analysis. Six ESTs that were nucleus-encoded exhibited differential expression patterns in the green wild-type bamboo relative to the albino mutant. These genes (exception being the Rubisco small subunit) were non-photosynthesis-related genes. The development of a specific SSH cDNA library in which most of the chloroplast-encoded or photosynthesis-related genes had been subtracted proved to be useful for studying the function of non-photosynthesis-related genes in the albino bamboo mutants with aberrant chloroplast genome. The combined use of this SSH library with microarray analysis will provide a powerful analytical tool for future studies of the bamboo genome.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-006-9105-3