Leptomyxa valladaresi n. sp. (Amoebozoa, Tubulinea, Leptomyxida), from Mount Teide, Tenerife, Spain

Leptomyxa valladaresi was isolated from soil in a pine forest on the southern flank of Mt Teide in Tenerife, Spain. It feeds on bacteria and on a range of other amoebae, and it was possible to establish bi-axenic cultures with L. valladaresi and Acanthamoeba. It is easily propagated on a E. coli als...

Full description

Saved in:
Bibliographic Details
Published in:Experimental parasitology 2017-12, Vol.183, p.85-91
Main Authors: De Obeso Fernandez Del Valle, Alvaro, Lorenzo-Morales, Jacob, Maciver, Sutherland K.
Format: Article
Language:English
Subjects:
Acanthamoeba - parasitology
Amoebozoa
DNA, Ribosomal - chemistry
Escherichia coli
Forests
Leptomyxida
Lobosea - classification
Lobosea - genetics
Lobosea - physiology
Lobosea - ultrastructure
Locomotion
Phylogeny
RNA, Ribosomal, 18S - genetics
Soil - parasitology
Spain
Systematics
Yeasts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Leptomyxa valladaresi was isolated from soil in a pine forest on the southern flank of Mt Teide in Tenerife, Spain. It feeds on bacteria and on a range of other amoebae, and it was possible to establish bi-axenic cultures with L. valladaresi and Acanthamoeba. It is easily propagated on a E. coli also. 18S rDNA gene sequence analysis suggests that it is most closely related to Leptomyxa variabilis, however this amoeba differs in important detail. L. valladaresi is primarily mononucleate whereas L. variabilis is multinucleate. L. valladaresi is a larger amoeba and although the cysts are similar in size, there is no sign of the pore-like structures described in L. variabilis cysts. L. valladaresi can adopt a rapid monopodal and tubular morphology similar to that described for L. neglecta and Rhizamoeba matisi, and is never reticulated as larger L. variabilis individuals tend to be. The mean generation time was found to be 18 h, in line with amoebae of this size. Like other members of the genus, L. valladaresi is reported to harbour intracellular, presumably endosymbiotic bacteria, and a Delftia sp has been identified by 16S PCR a bacterium which is also known to grow within Acanthamoeba. The availability of this easily cultured species will help to characterize of this little studied genus and family and their relationship with bacteria, both prey and symbionts. [Display omitted] •A new species of Leptomyxa is described.•Forms two different locomotory morphology one cylindrical the other flattened and fan like.•Feeds on bacteria and Acanthamoeba.•Contains endosymbiont bacteria which may produce geosmin.
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2017.09.017