Effects of polysaccharides from the base of Flammulina Velutipes stipe on growth of murine RAW264.7, B16F10 and L929 cells

A polysaccharide (FVSP) was isolated from the base of Flammuliana Velutipes stipe, and FVSP was further purified by DEAE-cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography to obtain three fractions named FVSP-1, FVSP-2 and FVSP-3. Then their activation of macrophage cell RA...

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Bibliographic Details
Published in:International journal of biological macromolecules 2018-02, Vol.107 (Pt B), p.2150-2156
Main Authors: Chen, Gui-tang, Fu, Ya-xin, Yang, Wen-jian, Hu, Qiu-hui, Zhao, Li-yan
Format: Article
Language:English
Subjects:
Animals
Antitumor activity
B16F10
Cell Proliferation - drug effects
Coculture Techniques
Flammulina - chemistry
Flammulina velutipes
L929
Macrophages - cytology
Macrophages - drug effects
Macrophages - metabolism
Melanoma, Experimental - pathology
Mice
Neutral Red - metabolism
Phagocytosis - drug effects
Polysaccharides
Polysaccharides - pharmacology
RAW 264.7 Cells
RAW264.7
Spectrophotometry, Infrared
Spectrophotometry, Ultraviolet
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Summary:A polysaccharide (FVSP) was isolated from the base of Flammuliana Velutipes stipe, and FVSP was further purified by DEAE-cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography to obtain three fractions named FVSP-1, FVSP-2 and FVSP-3. Then their activation of macrophage cell RAW 264.7 and anti-proliferative effects to the murine melanoma B16F10 and fibroblasts L929 cells were evaluated by using the cell model experiments. The results indicated that the polysaccharide fractions could increase the proliferation and phagocytic activity of macrophage significantly and play an inhibited effect on the cancer cells. Moreover, the anti-proliferative activities of FVSPs increased with the participation of the antitumor factors induced from macrophage by polysaccharides fractions. Taken together, these results indicated that three polysaccharides fractions from the base of F. Velutipes stipe may be useful as potent antitumor agents for the prevention of tumorigenesis.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2017.10.090