Prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks determined by polymerase chain reaction with two pairs of primers detecting 16S rRNA and ankA genes

A total of 684 Ixodes ricinus ticks (321 nymphs, 184 males, and 179 females) were collected by flagging lower vegetation in 6 forest districts located on the territory of Lublin province (eastern Poland). Ticks were examined by polymerase chain reaction (PCR) method for the presence of Anaplasma pha...

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Bibliographic Details
Published in:Annals of Agricultural and Environmental Medicine 2007, Vol.14 (2), p.281-285
Main Authors: Chmielewska-Badora, Jolanta, Zwoliński, Jacek, Cisak, Ewa, Wójcik-Fatla, Angelina, Buczek, Alicja, Dutkiewicz, Jacek
Format: Article
Language:English
Subjects:
Adult
Anaplasma
Anaplasma phagocytophilum
Anaplasma phagocytophilum - genetics
Anaplasma phagocytophilum - immunology
Anaplasma phagocytophilum - isolation & purification
Animals
Antibodies, Bacterial - blood
Antigens, Bacterial - chemistry
Antigens, Bacterial - genetics
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Base Sequence
Bites and Stings
Case reports
DNA Primers
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Ehrlichiosis - epidemiology
Female
Forestry
Humans
Immunofluorescence
Infections
Insect bites
Ixodes - microbiology
Ixodes ricinus
Ixodidae
Male
Nymph - microbiology
Occupational Diseases - epidemiology
Occupational exposure
Poland - epidemiology
Polymerase chain reaction
Polymerase Chain Reaction - methods
Primers
Risk Factors
RNA, Ribosomal, 16S - chemistry
RNA, Ribosomal, 16S - genetics
rRNA 16S
Seroepidemiologic Studies
Tick-Borne Diseases - epidemiology
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Summary:A total of 684 Ixodes ricinus ticks (321 nymphs, 184 males, and 179 females) were collected by flagging lower vegetation in 6 forest districts located on the territory of Lublin province (eastern Poland). Ticks were examined by polymerase chain reaction (PCR) method for the presence of Anaplasma phagocytophilum DNA with two pairs of primers: EHR521/EHR747 for detecting 16S rRNA gene, and LA6/LA1 for detecting ankA gene. To study the relationship between infection in ticks and people occupationally exposed to tick bite, blood serum samples of 261 forestry workers employed in the same forest districts were examined by immunofluorescence method for the presence of specific antibodies against A. phagocytophilum. A total of 70 ticks out of 684 examined (10.2%) showed the presence of A. phagocytophilum 16S rRNA gene. The prevalence of infection was significantly dependent on tick's stage (chi-square=49.2, p
ISSN:1232-1966
1898-2263