Prevalence of Streptococcus suis Genotypes in Wild Boars of Northwestern Germany

• Published in: Applied and Environmental Microbiology 2007-02, Vol.73 (3), p.711-717
• Main Authors: Baums, Christoph G, Verkühlen, Gerd Josef, Rehm, Thomas, Silva, Luciana M.G, Beyerbach, Martin, Pohlmeyer, Klaus, Valentin-Weigand, Peter
• Format: Article
• Language: English
• Subjects: Animals; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Genotype; Genotype & phenotype; Germany - epidemiology; Hogs; Humans; Meningitis, Bacterial - epidemiology; Meningitis, Bacterial - microbiology; Meningitis, Bacterial - veterinary; Microbial Ecology; Microbiology; Molecular Sequence Data; Palatine Tonsil - microbiology; Polymorphism; Prevalence; Sequence Analysis, DNA; Serotyping; Streptococcal Infections - epidemiology; Streptococcal Infections - microbiology; Streptococcal Infections - veterinary; Streptococcus suis; Streptococcus suis - classification; Streptococcus suis - genetics; Streptococcus suis - isolation & purification; Studies; Sus scrofa - microbiology; Swine Diseases - epidemiology; Swine Diseases - microbiology
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.01800-06

Invasive serotype 2 (cps2⁺) strains of Streptococcus suis cause meningitis in pigs and humans. Four case reports of S. suis meningitis in hunters suggest transmission of S. suis through the butchering of wild boars. Therefore, the objective of this study was to investigate the prevalence of potentially human-pathogenic S. suis strains in wild boars. S. suis was isolated from 92% of all tested tonsils (n = 200) from wild boars. A total of 244 S. suis isolates were genotyped using PCR assays for the detection of serotype-specific genes, the hemolysin gene sly, and the virulence-associated genes mrp and epf. The prevalence of the cps2⁺ genotype among strains from wild boars was comparable to that of control strains from domestic pig carriers. Ninety-five percent of the cps2⁺ wild boar strains were positive for mrp, sly, and epf*, the large variant of epf. Interestingly, epf* was significantly more frequently detected in cps2⁺ strains from wild boars than in those from domestic pigs; epf* is also typically found in European S. suis isolates from humans, including a meningitis isolate from a German hunter. These results suggest that at least 10% of wild boars in Northwestern Germany carry S. suis strains that are potentially virulent in humans. Additional amplified fragment length polymorphism analysis supported this hypothesis, since homogeneous clustering of the epf* mrp⁺ sly⁺ cps2⁺ strains from wild boars with invasive human and porcine strains was observed.