Different mutational function of low‐ and high‐linear energy transfer heavy‐ion irradiation demonstrated by whole‐genome resequencing of Arabidopsis mutants

Summary Heavy‐ion irradiation is a powerful mutagen that possesses high linear energy transfer (LET). Several studies have indicated that the value of LET affects DNA lesion formation in several ways, including the efficiency and the density of double‐stranded break induction along the particle path...

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Bibliographic Details
Published in:The Plant journal : for cell and molecular biology 2017-12, Vol.92 (6), p.1020-1030
Main Authors: Kazama, Yusuke, Ishii, Kotaro, Hirano, Tomonari, Wakana, Taeko, Yamada, Mieko, Ohbu, Sumie, Abe, Tomoko
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis - genetics
Arabidopsis - physiology
Arabidopsis - radiation effects
Arabidopsis thaliana
Beams (radiation)
chromosomal rearrangement
Chromosome Aberrations - radiation effects
Chromosome rearrangements
Deoxyribonucleic acid
DNA
Energy transfer
Gene Rearrangement
Gene sequencing
Genetics
Genomes
Heavy Ions
heavy‐ion beam
High-Throughput Nucleotide Sequencing
Ion irradiation
Ions
Irradiation
linear energy transfer
Linear energy transfer (LET)
Linear Energy Transfer - radiation effects
Mutagenesis
Mutants
Mutation
mutation spectrum
Next-generation sequencing
Radiation, Ionizing
Sequence Analysis, DNA
Sequence Deletion - radiation effects
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Summary:Summary Heavy‐ion irradiation is a powerful mutagen that possesses high linear energy transfer (LET). Several studies have indicated that the value of LET affects DNA lesion formation in several ways, including the efficiency and the density of double‐stranded break induction along the particle path. We assumed that the mutation type can be altered by selecting an appropriate LET value. Here, we quantitatively demonstrate differences in the mutation type induced by irradiation with two representative ions, Ar ions (LET: 290 keV μm−1) and C ions (LET: 30.0 keV μm−1), by whole‐genome resequencing of the Arabidopsis mutants produced by these irradiations. Ar ions caused chromosomal rearrangements or large deletions (≥100 bp) more frequently than C ions, with 10.2 and 2.3 per mutant genome under Ar‐ and C‐ion irradiation, respectively. Conversely, C ions induced more single‐base substitutions and small indels (
ISSN:0960-7412
1365-313X
DOI:10.1111/tpj.13738