Applied multiplex allele specific PCR to detect second-line drug resistance among multidrug-resistant tuberculosis in China

Rapid detection of resistance to the second-line drugs is essential for early initiation of appropriate anti-tubercular treatment regimen among multi-drug tuberculosis (MDR-TB). In this study, we applied a multiplex allele-specific PCR (MAS-PCR) to identify the mutations on codons 90 and 94 of gyrA...

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Bibliographic Details
Published in:Tuberculosis (Edinburgh, Scotland) Scotland), 2017-12, Vol.107, p.1-4
Main Authors: Liang, Ya-ping, Chen, Yan, Xiao, Tong-yang, Xia, Qiang, Liu, Hai-can, Zhao, Xiu-qin, Zeng, Chun-yan, Zhao, Li-li, Wan, Kang-lin
Format: Article
Language:English
Subjects:
Antitubercular Agents - therapeutic use
Bacteriological Techniques
China
DNA Gyrase - genetics
DNA Mutational Analysis - methods
Drug Resistance, Multiple, Bacterial - genetics
Genotype
Humans
Kanamycin
Kanamycin - therapeutic use
Kanamycin Resistance - genetics
Microbial Sensitivity Tests
Multidrug-resistant tuberculosis
Multiplex allele specific PCR
Multiplex Polymerase Chain Reaction
Mutation
Mycobacterium tuberculosis - drug effects
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - pathogenicity
Ofloxacin
Ofloxacin - therapeutic use
Predictive Value of Tests
Ribosomal Proteins - genetics
Tuberculosis, Multidrug-Resistant - diagnosis
Tuberculosis, Multidrug-Resistant - drug therapy
Tuberculosis, Multidrug-Resistant - microbiology
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Summary:Rapid detection of resistance to the second-line drugs is essential for early initiation of appropriate anti-tubercular treatment regimen among multi-drug tuberculosis (MDR-TB). In this study, we applied a multiplex allele-specific PCR (MAS-PCR) to identify the mutations on codons 90 and 94 of gyrA and nucleotide 1401 of rrs for detecting ofloxacin (OFX) and kanamycin (KAN) resistance in 139 MDR-TB isolates from China. Using the traditional phenotypic method as the reference, MAS-PCR detected resistance to OFX and KAN with sensitivities of 67.3% and 76.5%, respectively, and specificities of 100.0%. Therefore, MAS-PCR assays can be used for rapid detection of second-line drug resistance among MDR-TB in China, enabling early administration of appropriate treatment regimens to the affected MDR-TB patients.
ISSN:1472-9792
1873-281X
DOI:10.1016/j.tube.2017.07.010