A tracking algorithm for cell motility assays in CMOS systems

This work proposes a method for the study and real-time monitoring of a single cell on a 2D electrode matrix, of great interest in cell motility assays and in the characterization of cancer cell metastasis. A CMOS system proposal for cell location based on occupation maps data generated from Electri...

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Bibliographic Details
Main Authors: Martinez-Gomez, C., Olmo, A., Huertas, G., Perez, P., Maldonado-Jacobi, A., Yufera, A.
Format: Conference Proceeding
Language:English
Subjects:
Cancer
Electrodes
Fluorescence
Indexes
Real-time systems
Sensors
Two dimensional displays
Online Access:Get full text
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Summary:This work proposes a method for the study and real-time monitoring of a single cell on a 2D electrode matrix, of great interest in cell motility assays and in the characterization of cancer cell metastasis. A CMOS system proposal for cell location based on occupation maps data generated from Electrical Cell-substrate Impedance Spectroscopy (ECIS) has been developed. From experimental assays data, an algorithm based on the analysis of the eight nearest neighbours has been implemented to find the cell center of mass. The path followed by a cell, proposing a Brownian route, has been simulated with the proposed algorithm. The presented results give an accuracy over 95% in the determination of the coordinates (x, y) from the expected cell center of mass.
ISSN:1557-170X
2694-0604
DOI:10.1109/EMBC.2017.8036954