Investigation on human serum albumin and Gum Tragacanth interactions using experimental and computational methods

The study on the interaction of human serum albumin and Gum Tragacanth, a biodegradable bio-polymer, has been undertaken. For this purpose, several experimental and computational methods were used. Investigation of thermodynamic parameters and mode of interactions were carried out using Fluorescence...

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Bibliographic Details
Published in:International journal of biological macromolecules 2018-02, Vol.107 (Pt B), p.2525-2533
Main Authors: Moradi, Sajad, Taran, Mojtaba, Shahlaei, Mohsen
Format: Article
Language:English
Subjects:
Computational Biology - methods
Gum Tragacanth
Humans
Molecular Docking Simulation
Plant Gums - chemistry
Plant Gums - metabolism
Protein conformation
Protein-biopolymer interaction
Rheology
Serum Albumin, Human - chemistry
Serum Albumin, Human - metabolism
Spectrometry, Fluorescence
Spectroscopy, Fourier Transform Infrared
Thermodynamics
Tragacanth - chemistry
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Summary:The study on the interaction of human serum albumin and Gum Tragacanth, a biodegradable bio-polymer, has been undertaken. For this purpose, several experimental and computational methods were used. Investigation of thermodynamic parameters and mode of interactions were carried out using Fluorescence spectroscopy in 300 and 310K. Also, a Fourier transformed infrared spectra and synchronous fluorescence spectroscopy was performed. To give detailed insight of possible interactions, docking and molecular dynamic simulations were also applied. Results show that the interaction is based on hydrogen bonding and van der Waals forces. Structural analysis implies on no adverse change in protein conformation during binding of GT. Furthermore, computational methods confirm some evidence on secondary structure enhancement of protein as a presence of combining with Gum Tragacanth.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2017.10.129