Metagenomic Analysis of Gingival Sulcus Microbiota and Pathogenesis of Periodontitis Associated with Type 2 Diabetes Mellitus

• Published in: Bulletin of experimental biology and medicine 2017-10, Vol.163 (6), p.718-721
• Main Authors: Babaev, E. A., Balmasova, I. P., Mkrtumyan, A. M., Kostryukova, S. N., Vakhitova, E. S., Il’ina, E. N., Tsarev, V. N., Gabibov, A. G., Arutyunov, S. D.
• Format: Article
• Language: English
• Subjects: Adult; Aggregatibacter actinomycetemcomitans - classification; Aggregatibacter actinomycetemcomitans - genetics; Aggregatibacter actinomycetemcomitans - isolation & purification; Analysis; Bacteria; Biofilms - growth & development; Biomedical and Life Sciences; Biomedicine; Case-Control Studies; Cell Biology; Chronic Periodontitis - complications; Chronic Periodontitis - microbiology; Chronic Periodontitis - pathology; Dental Plaque - complications; Dental Plaque - microbiology; Dental Plaque - pathology; Development and progression; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - microbiology; Diabetes Mellitus, Type 2 - pathology; Female; Gingiva - microbiology; Humans; Internal Medicine; Laboratory Medicine; Male; Medical research; Metagenome; Microbiota (Symbiotic organisms); Middle Aged; Pathology; Periodontitis; Porphyromonas gingivalis - classification; Porphyromonas gingivalis - genetics; Porphyromonas gingivalis - isolation & purification; RNA; RNA, Ribosomal, 16S - genetics; Treponema denticola - classification; Treponema denticola - genetics; Treponema denticola - isolation & purification; Type 2 diabetes
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-017-3888-6

Biofilm of the gingival sulcus from 22 patients with type 2 diabetes mellitus and periodontitis, 30 patients with periodontitis not complicated by diabetes mellitus (reference group), and 22 healthy volunteers without signs of gingival disease (control group) was studied by quantitative PCR. Quantitative analysis for the content of P. gingivalis , T. forsythia , A. ctinomycetemcomitans , T. denticola , P. intermedia , F. nucleatum/periodonticum , and P. endodontalis in the dental plaque was performed with a Dentoscreen kit. The presence of other bacterial groups was verified by metagenomic sequencing of the 16S rRNA gene to evaluate some specific features of the etiological factor for periodontitis in type 2 diabetes mellitus. Specimens of the Porphiromonadaceae and Fusobacteriaceae families were characterized by an extremely high incidence in combined pathology. The amount of Sphingobacteriaceae bacteria in the biofilm was shown to decrease significantly during periodontitis. Metagenomic analysis confirmed the pathogenic role of microbiota in combined pathology, as well as the hypothesis on a possible influence of periodontitis on the course and development of type 2 diabetes mellitus.